Uses of Growth and Differentiation Factor 8 (GDF-8)
First Claim
1. A method for reducing the immunogenicity of cells in vitro, the method comprising sing cells to growth and differentiation factor 8 (GDF-8), wherein the cells are selected from the group consisting of mesenchymal stem cells (MSC), cells obtained by differentiation of MSC, cells of osteocytic lineage, cells of chondrocytic lineage, cells of adipocytic lineage, cells of myocytic lineage, cells of tendonocytic lineage, cells of fibroblastic lineage, and cells of stromogenic lineage.
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Abstract
The present invention relates to uses of growth and differentiation factor 8 (GDF-8) in vitro or in vivo for reducing the immunogenicity or risk of rejection of cells such as in particular mesenchymal stem cells (MSC), tissues or materials. The present invention further relates to methods for differentiating MSC in vitro or ex vivo into osteoprogenitors or osteoblastic cells or a cell population comprising osteoprogenitors and/or osteoblastic cells using FGF-2 and GDF-8. In addition, the present invention relates to osteoprogenitors or osteoblastic cells or a cell population comprising osteoprogenitors and/or osteoblastic cells obtainable by such methods and to the osteoprogenitors or osteoblastic cells or a cell population comprising osteoprogenitors and/or osteoblastic cells for use in the treatment of musculoskeletal diseases.
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Citations
23 Claims
- 1. A method for reducing the immunogenicity of cells in vitro, the method comprising sing cells to growth and differentiation factor 8 (GDF-8), wherein the cells are selected from the group consisting of mesenchymal stem cells (MSC), cells obtained by differentiation of MSC, cells of osteocytic lineage, cells of chondrocytic lineage, cells of adipocytic lineage, cells of myocytic lineage, cells of tendonocytic lineage, cells of fibroblastic lineage, and cells of stromogenic lineage.
- 10. A method for reducing the immunogenicity of cells with GDF-8 in vivo in a subject, the method comprising exposing cells in a subject to GDF-8 in vivo, wherein the cells are selected from the group consisting of MSC, cells obtained by differentiation of MSC, cells of osteocytic lineage, cells of chondrocytic lineage, cells of adipocytic lineage, cells of myocytic lineage, cells of tendonocytic lineage, cells of fibroblastic lineage, and cells of stromogenic lineage.
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14. A method for treating a musculoskeletal disease in a patient in need thereof, the method comprising administering a combination of GDF-8 and cells to the patient,
wherein the cells are selected from the group consisting of MSC, cells obtained by differentiation of MSC, cells of osteocytic lineage, cells of chondrocytic lineage, cells of adipocytic lineage, cells of myocytic lineage, cells of tendonocytic lineage, cells of fibroblastic lineage, and cells of stromogenic lineage.
- 18. A method of reducing the risk of rejection by a patient of a material administered, implanted, or transplanted into the patient, the method comprising co-administering GDF-8 and the material to the patient.
- 20. A pharmaceutical composition comprising a material to be administered, implanted, or transplanted into a patient and GDF-8, and optionally further comprising one or more pharmaceutically acceptable excipients.
Specification