CORN PLANT MON88017 AND COMPOSITIONS AND METHODS FOR DETECTION THEREOF

• US 20140287406A1
• Filed: 02/26/2014
• Published: 09/25/2014
• Est. Priority Date: 12/15/2003
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Beazley, Kim A., Coombe, Timothy R., Groth, Mark E., Hinchey, Terri B., Pershing, Jay C., Vaughn, Ty T., Zhang, Bei
• Granted Patent: US 9,556,492 B2
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention provides a corn plant designated MON88017 and DNA compositions contained therein. Also provided are assays for detecting the presence of the corn plant MON88017 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.

Claims

1-3. -3. (canceled)

4. A method of detecting the presence of DNA corresponding to corn event MON88017 in a sample, the method comprising:

(a) contacting the sample with a DNA primer pair, wherein said DNA primer pair comprises a first DNA molecule and a second DNA molecule that function as DNA primers when used together in a DNA amplification method to produce an amplicon diagnostic for corn event MON88017 DNA; and

(b) performing a polynucleic acid amplification reaction, thereby producing an amplicon; and

(c) detecting said amplicon,

wherein said amplicon comprises SEQ ID NO;

1 or SEQ ID NO;

2.

6. The method of claim 4, wherein said DNA primer pair comprises SEQ ID NO:

6 and SEQ ID NO;

7.

5. (canceled)

7. A method of detecting the presence of a DNA corresponding to corn event MON88017 in a sample, the method comprising:

(a) contacting the sample comprising DNA with a probe that hybridizes under stringent hybridization conditions with DNA from corn event MON88017 and does not hybridize under the stringent hybridization conditions with a control corn plant genomic DNA, wherein said probe comprises a nucleotide sequence homologous or complementary to SEQ ID NO;

1 or SEQ ID NO;

2;

(b) subjecting the sample and probe to stringent hybridization conditions; and

(c) detecting hybridization of the probe to the DNA.

8. (canceled)

9. A method of determining the zygosity of a corn plant comprising event MON88017 comprising:

(a) contacting a sample comprising corn DNA with a primer set that when used in a nucleic-acid amplification reaction with genomic DNA from corn event MON88017, produces a first amplicon that is diagnostic for corn event MON88017, or that when used in a nucleic-acid amplification reaction with genomic DNA from corn plants that do not comprise corn event MON88017 produces a second amplicon comprising the native corn genomic DNA region spanning the insertion location of the transgene of corn event MON88017; and

(b) performing a nucleic acid amplification reaction, thereby producing the first amplicon or the second amplicon;

(c) detecting the first amplicon or the second amplicon; and

(d) comparing the first and second amplicons in the amplification reaction, wherein the presence of both amplicons indicates the sample is heterozygous for the transgene insertion and the presence of only said first amplicon indicates said sample is homozygous for the transgene insertion.

33. The method of claim 9, wherein the primer set comprises SEQ ID NO:

30, SEQ ID NO;

31, and SEQ ID NO;

32.

10-32. -32. (canceled)