METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF ORGANIC SUBSTANCES FROM FERMENTING MICROORGANISMS

• US 20140287473A1
• Filed: 03/22/2013
• Published: 09/25/2014
• Est. Priority Date: 03/22/2013
• Status: Abandoned Application

• Current Assignee: Danisco US, Inc. (International Flavors & Fragrances Incorporated)
• Original Assignee: Danisco US, Inc. (International Flavors & Fragrances Incorporated)
• Inventors: Li, Mian, Mitchinson, Colin, Steele, Landon M.
• Application Number:
  US13/848,950
• Publication Number:
  US 20140287473A1
• Time in Patent Office: Days
• US Class Current: 435/165
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates methods and compositions for producing an organic substance from fermenting microorganism using simultaneous saccharification and fermentation. One aspect of the invention provides producing alcohol by simultaneous saccharification and fermentation by combining a cellulosic substrate, and whole fermentation broth, and an ethanologenic microorganism under conditions conducive both to hydrolysis of cellulose to glucose and to conversion of glucose to alcohol.

Claims

1. A method of producing an organic substance by simultaneous saccharification and fermentation comprising:

(a) combining, in the absence of a supplemental nitrogen source, a cellulosic substrate, a whole fermentation broth and a fermenting microorganism; and

(b) incubating the cellulosic substrate, whole fermentation broth and fermenting microorganism under conditions conducive both to hydrolysis of cellulose to glucose and/or xylose and to conversion of glucose and/or xylose to organic substance.

2. The method of claim 1, wherein the cellulosic substrate comprises one or more cellulose source selected from the group consisting of wood, wood pulp, papermaking sludge, paper pulp waste streams, particle board, corn stover, corn fiber, corn cob, rice, paper and pulp processing waste, woody or herbaceous plants, fruit pulp, vegetable pulp, pumice, distillers grain, grasses, rice hulls, sugar cane bagasse, cotton, jute, hemp, flax, bamboo, sisal, abaca, straw, corn cobs, leaves, wheat straw, coconut hair, algae, switchgrass, and mixtures thereof.

3. The method of claim 1, wherein the cellulosic substrate is mechanically or chemically pretreated.

4. The method of claim 1, wherein the whole fermentation broth is prepared from fermentation of a filamentous fungi.

5. The method of claim 1, wherein the fermenting microorganism is a yeast or bacterial cell.

6. The method of claim 1, wherein the fermenting microorganism is an ethanologenic microorganism.

7. The method of claim 1, wherein the organic substance is alcohol.

8. The method of claim 1, wherein the organic substance is ethanol.

9. A reactive composition for production of an organic substance comprising a mixture of a cellulosic substrate, whole fermentation broth and fermenting microorganism, wherein the reactive composition is substantially free of supplemental nitrogen source.

10. The reactive composition of claim 9, wherein the cellulosic substrate comprises one or more cellulose source selected from the group consisting of wood, wood pulp, papermaking sludge, paper pulp waste streams, particle board, corn stover, corn fiber, corn cob, rice, paper and pulp processing waste, woody or herbaceous plants, fruit pulp, vegetable pulp, pumice, distillers grain, grasses, rice hulls, sugar cane bagasse, cotton, jute, hemp, flax, bamboo, sisal, abaca, straw, corn cobs, leaves, wheat straw, coconut hair, algae, switchgrass, and mixtures thereof.

11. The reactive composition of claim 9, wherein the cellulosic substrate is mechanically or chemically pretreated.

12. The reactive composition of claim 9, wherein the whole fermentation broth is a filamentous fungi whole fermentation broth.

13. The reactive composition of claim 9, wherein the fermenting microorganism is a yeast or bacterial cell.

14. The reactive composition of claim 9, wherein the fermenting microorganism is an ethanologenic microorganism.