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PRIMER SET, METHOD FOR AMPLIFYING TARGET NUCLEIC ACID SEQUENCE USING SAME, AND METHOD FOR DETECTING MUTATED NUCLEIC ACID USING SAME

  • US 20140295447A1
  • Filed: 09/07/2012
  • Published: 10/02/2014
  • Est. Priority Date: 09/08/2011
  • Status: Active Grant
First Claim
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1. A primer set for use in a method for isothermally amplifying a target nucleic acid sequence, the primer set comprising:

  • a first primer; and

    a second primer,wherein the first primer comprises, on the 3′

    side thereof, a sequence (A′

    ) that can hybridize to a sequence (A) on the 3′

    side of the target nucleic acid sequence,the second primer comprises, on the 3′

    side thereof, a sequence (B′

    ) that can hybridize to a sequence (B) on the 3′

    side of either a strand extended from the first primer or a strand complementary to the target nucleic acid sequence, andthe first primer and the second primer comprise, on the 5′

    sides thereof, sequences (C) that are substantially identical to each other.

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