BLOOD CELL ANALYZER AND BLOOD CELL ANALYZING METHOD

• US 20140295488A1
• Filed: 03/27/2014
• Published: 10/02/2014
• Est. Priority Date: 03/29/2013
• Status: Abandoned Application

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: KAWASHIMA, Yasuyuki, YAMADA, Kazuhiro, YOSHIKAWA, Keiko, KONISHI, Yusuke
• Application Number:
  US14/227,795
• Publication Number:
  US 20140295488A1
• Time in Patent Office: Days
• US Class Current: 435/34
• CPC Class Codes: G01N 15/0205 by optical means
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 2015/1402 Data analysis by thresholding or gating operations performed on the acquired signals or stored data
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 33/56972 White blood cells

Abstract

A blood cell analyzer comprising a flow cell configured to flow a measurement specimen containing blood cells, a first light source configured to emit light having a first wavelength, a second light source configured to emit light having a second wavelength different from the first wavelength, a first light receiving portion configured to receive first scattered light generated by irradiating the blood cells in the measurement specimen with light from the first light source, a second light receiving portion configured to receive second scattered light generated by irradiating the blood cells in the measurement specimen with light from the second light source, and a control section configured to classify at least white blood cells from the blood cells contained in the measurement specimen, based on a detection signal output from the first light receiving portion and a detection signal output from the second light receiving portion.

Claims

1. A blood cell analyzer comprising:

a flow cell configured to flow a measurement specimen containing blood cells;

a first light source configured to emit light having a first wavelength on the measurement specimen;

a second light source configured to emit light having a second wavelength different from the first wavelength on the measurement specimen;

a first light receiving portion configured to receive first scattered light generated by irradiating the blood cells in the measurement specimen with light from the first light source;

a second light receiving portion configured to receive second scattered light generated by irradiating the blood cells in the measurement specimen with light from the second light source; and

a control section configured to classify at least white blood cells from the blood cells contained in the measurement specimen, based on a detection signal output from the first light receiving portion and a detection signal output from the second light receiving portion.

2. The blood cell analyzer according to claim 1, further comprising:

a specimen preparing section configured to prepare the measurement specimen without hemolyzing red blood cells, from a blood sample containing the blood cells.

3. The blood cell analyzer according to claim 2, wherein

the specimen preparing section prepares the measurement specimen without staining the white blood cells.

4. The blood cell analyzer according to claim 2, further comprising:

a third light receiving portion configured to receive fluorescence obtained by irradiating the blood cells passing through the flow cell with light, wherein

the specimen preparing section is configured to mix the blood sample, and a fluorescence labeled antibody that specifically reacts with a specific cell surface antigen; and

the control section configured to further classify the white blood cells contained in the measurement specimen based on a detection signal output from the third light receiving portion.

5. The blood cell analyzer according to claim 1, wherein

an absorption coefficient of hemoglobin of the first wavelength is different from an absorption coefficient of hemoglobin of the second wavelength.

6. The blood cell analyzer according to claim 1, wherein

the first light source is a semiconductor laser light source configured to irradiate light having the first wavelength of greater than or equal to 400 nm and smaller than or equal to 435 nm.

7. The blood cell analyzer according to claim 1, wherein

the second light source is a semiconductor laser light source configured to irradiate a light having the second wavelength of greater than or equal to 610 nm and smaller than or equal to 750 nm.

8. The blood cell analyzer according to claim 1, wherein

the first light receiving portion is configured to receive first forward scattered light generated by irradiating the blood cells with light having the first wavelength;

and

the second light receiving portion is configured to receive second forward scattered light generated by irradiating the blood cells with the light having the second wavelength.

9. The blood cell analyzer according to claim 1, wherein

the control section is configured to acquire the detection signal containing an intensity of the first scattered light and an intensity of the second scattered light for each of blood cells flowing through the flow cell, and compare the intensity of the first scattered light and the intensity of the second scattered light of each of blood cells with a predetermined intensity range to classify at least the white blood cells from the blood cells contained in the measurement specimen.

10. The blood cell analyzer according to claim 9, further comprising:

a display section configured to display an image;

wherein

the control section is configured to generate a scattergram having the intensity of the first scattered light and the intensity of the second scattered light as two axes based on information associated with the intensity of the first scattered light and information associated with the intensity of the second scattered light acquired for each of blood cells flowing through the flow cell; and

the control section is configured to display the generated scattergram on the display section.

11. The blood cell analyzer according to claim 1, wherein

the control section is configured to further classify the white blood cells in the measurement specimen into a plurality of types.

12. The blood cell analyzer according to claim 1, wherein

the control section is configured to exclude, from an analyzing target, a detection signal that does not satisfy a first condition for classifying the white blood cells, of the detection signals of each blood cell output from the first light receiving portion and the second light receiving portion.

13. The blood cell analyzer according to claim 12, wherein

the control section is configured to exclude the detection signal that does not satisfy the first condition from an acquiring target of analysis data.

14. The blood cell analyzer according to claim 1, wherein

the control section is configured to perform classification of the white blood cells and classification of other blood cells other than the white blood cells using the same measurement specimen.

15. The blood cell analyzer according to claim 14, wherein

the control section is configured to acquire the analysis data from the detection signal based on a first condition for classifying the white blood cells when classifying the white blood cells;

and

the control section is configured to acquire the analysis data from the detection signal based on a second condition different from the first condition when classifying the other blood cells other than the white blood cells.

16. The blood cell analyzer according to claim 15, wherein

the control section is configured to switch the acquiring condition of the analysis data between the first condition and the second condition in the middle of a series of measurements with respect to the measurement specimen.

17. A blood cell analyzing method comprising:

flowing a measurement specimen containing blood cells through a flow cell;

detecting first scattered light obtained by irradiating the blood cells passing through the flow cell with light having a first wavelength to acquire a first detection signal;

detecting a second scattered light obtained by irradiating the blood cells passing through the flow cell with light having a second wavelength to acquire a second detection signal; and

classifying at least white blood cells from the blood cells contained in the measurement specimen, based on the first detection signal and the second detection signal.

18. A blood cell analyzing method comprising:

mixing a specimen containing blood cells and a fluorescence labeled antibody that specifically reacts with a cell surface antigen, and preparing a measurement specimen without performing a process of removing red blood cells;

flowing the prepared measurement specimen through a flow cell;

detecting first scattered light obtained by irradiating the blood cells passing through the flow cell with light having a first wavelength to acquire a first detection signal;

detecting second scattered light obtained by irradiating the blood cells passing through the flow cell with light having a second wavelength to acquire a second detection signal;

detecting fluorescence obtained by irradiating the blood cells passing through the flow cell with light having a predetermined wavelength to acquire a third detection signal; and

classifying white blood cells contained in the measurement specimen for a cell surface antigen, based on the first detection signal, the second detection signal, and the third detection signal.

19. The blood cell analyzing method according to claim 18, wherein

the light having the predetermined wavelength is the light having the first wavelength or the light having the second wavelength.

20. The blood cell analyzing method according to claim 18, comprising:

mixing the specimen with a plurality of types of fluorescence labeled antibodies that specifically react with each of a plurality of types of cell surface antigens and that excite fluorescence having different wavelengths from each other to prepare the measurement specimen;

detecting the fluorescence based on each fluorescence labeled antibody, respectively, to acquire a plurality of types of third detection signals; and

classifying white blood cells contained in the measurement specimen for a cell surface antigen, based on the first detection signal, the second detection signal, and the plurality of types of third detection signals.