NOVEL PD1 ISOFORMS, AND USES THEREOF FOR POTENTIATING IMMUNE RESPONSES
First Claim
1. An isolated PD1 protein isoform having a deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO:
- 2, or a fragment of the PD1 protein isoform, wherein the fragment has at least 30 amino acids and wherein the fragment has said deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO;
2.
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Accused Products
Abstract
In one embodiment, the present invention provides a new isoform of human PD1 (Δ42PD1) that contains a 42-nucleotide in-frame deletion located at exon 2 domain. Δ42PD1 does not engage PD-L1/PD-L2, and can induce the production of pro-inflammatory cytokines In one embodiment, Δ42PD1 can be used as an intramolecular adjuvant to develop a fusion DNA vaccine for enhancing antigen-specific CD8+ T cell immunity and for prevention of pathogenic infection and/or cancer. In one embodiment, soluble Δ42PD1 protein could be a therapeutic target for autoimmune diseases. In other embodiments, proteins or peptides or nucleic acids encoding proteins or peptides containing Δ42PD1 could be used as immunogens for developing antibodies binding specifically to Δ42PD1. In yet another embodiment, neutralizing antibodies could block sΔ42PD1 function and accordingly could be used as treatment for autoimmune disorders.
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Citations
20 Claims
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1. An isolated PD1 protein isoform having a deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO:
- 2, or a fragment of the PD1 protein isoform, wherein the fragment has at least 30 amino acids and wherein the fragment has said deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO;
2. - View Dependent Claims (2, 3, 4, 7, 8, 20)
- 2, or a fragment of the PD1 protein isoform, wherein the fragment has at least 30 amino acids and wherein the fragment has said deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO;
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5. A nucleic acid molecule encoding:
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a PD1 protein isoform having a deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO;
2;
ora fragment of the PD1 protein isoform wherein the fragment has at least 30 amino acids, wherein the fragment has said deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO;
2. - View Dependent Claims (6, 11, 12, 13, 14, 15, 17, 18)
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9. A fusion protein comprising:
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a PD1 protein isoform having a deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO;
2;
ora fragment of the PD1 protein isoform wherein the fragment has at least 30 amino acids, wherein the fragment has said deletion of 14 amino acids at positions corresponding to amino acids 26-39 of the wild-type PD1 protein having an amino acid sequence of SEQ ID NO;
2. - View Dependent Claims (10, 16, 19)
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Specification