Method of myceliating coffee
2 Assignments
0 Petitions
Accused Products
Abstract
A method of creating an extract of myceliated agricultural product for human consumption includes providing an agricultural substrate such as rice, where the agricultural substrate has been inoculated by liquid media comprising an aliquot of culture derived from liquid-state fermentation. The culture being selected from the group consisting of Basidiomycota and Ascomycota fungi. Next, the step of enabling mycelium growth on the substrate by controlling temperature, humidity and sterility of the environment. After mycelium growth on the substrate reaches a desired stage, then the step of boiling the substrate in water and separating the water-substrate mixture into aqueous component and non-aqueous components creates an extraction.
27 Citations
61 Claims
-
1-37. -37. (canceled)
-
38. A method for myceliating coffee beans, comprising:
-
sterilizing the coffee beans under pressure; providing a fungal liquid tissue culture grown in a media comprising a fruit-based and vegetable-based liquid-state media in the absence of an additional nitrogen source; inoculating the sterilized coffee beans with an aliquot of the fungal liquid tissue culture, the fungal liquid tissue culture including fungal hyphae having conglomerations of between 10 microns to 10 millimeters in diameter; and enabling the fungal culture to grow fungal mycelium on the coffee beans to prepare the myceliated coffee beans. - View Dependent Claims (39, 40, 41, 42, 44, 54)
-
-
43. (canceled)
-
45. A method for myceliating coffee beans, comprising:
-
sterilizing the coffee beans; providing a fungal liquid tissue culture comprising Ganoderma lucidum grown in a media comprising a fruit-based and vegetable-based liquid-state media in the absence of an additional nitrogen source; inoculating the sterilized coffee beans with an aliquot of the fungal liquid tissue culture, the fungal culture including an aliquot of conglomerations of fungal hyphae sized between 10 microns to 10 millimeters in diameter; enabling the fungal culture to grow fungal mycelium on the coffee beans and thereby produce components selected from the group consisting of;
glycoproteins, polysaccharides and triterpenes; andharvesting the coffee beans when the fungal mycelium reaches a desired state. - View Dependent Claims (46, 47, 48, 49, 50, 51, 55, 57)
-
-
52. A method for myceliating coffee beans, comprising:
-
sterilizing the coffee beans; providing a fungal liquid tissue culture comprising Cordyceps sinensis grown in a media comprising a fruit-based and vegetable-based liquid-state media in the absence of an additional nitrogen source; inoculating the sterilized coffee beans with an aliquot of the fungal liquid tissue culture having conglomerations of fungal hyphae sized between 10 microns to 10 millimeters in diameter; and enabling the fungal culture to grow fungal mycelium on the coffee beans and produce a component selected from of the group consisting of;
Cordycepic acid, Adenosine, D-Mannitol, and Cordycepinadenosine. - View Dependent Claims (53, 56, 58)
-
-
59. A method for myceliating stevia and green tea, comprising:
-
sterilizing the stevia or green tea under pressure; providing a fungal liquid tissue culture; inoculating the sterilized stevia or green tea with an aliquot of the fungal liquid tissue culture; and enabling the fungal culture to grow fungal mycelium on the stevia or green tea to prepare the myceliated stevia or green tea.
-
-
60. The method of claim 60, wherein the fungal liquid tissue culture is grown in a media comprising a fruit-based and vegetable-based liquid-state media in the absence of an additional nitrogen source.
-
61. The method of claim 61, wherein the fungal liquid tissue culture is grown in a media comprising a fruit-based and vegetable-based liquid-state media in the absence of an additional carbon source.
Specification