PROCESS FOR PRODUCING CELLULASES USING A FILAMENTOUS FUNGUS SUITABLE FOR A FERMENTER, HAVING A LOW VOLUMETRIC OXYGEN TRANSFER COEFFICIENT KLA

• US 20140302587A1
• Filed: 08/02/2012
• Published: 10/09/2014
• Est. Priority Date: 08/19/2011
• Status: Active Application

• Current Assignee: Agro industrie Recherches et Développements, IFP Energies Nouvelles, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement
• Original Assignee: Agro industrie Recherches et Développements, IFP Energies Nouvelles, Institut National de La Recherche Agronomique
• Inventors: Ben Chaabane, Fadhel, Cohen, Celine, Jourdier, Etienne, Chaussepied, Bernard
• Application Number:
  US14/239,690
• Publication Number:
  US 20140302587A1
• Time in Patent Office: Days
• US Class Current: 435/209
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)

Abstract

The present invention concerns a process for the production of cellulases using a strain of a filamentous fungus in a stirred, aerated bioreactor, comprising at least two growth steps:

• • the first growth step in the presence of at least one carbonaceous growth substrate in batch phase with a concentration of carbonaceous growth substrate in the range 10 to 80 g/L;
  • a second step for growth and production in the presence of at least one inducer carbonaceous substrate in fed batch phase in the presence of a limiting flow of carbon source in the range 50 to 140 mg per gram of cells per hour.

Claims

1. A process for the production of cellulases using a strain belonging to a filamentous fungus in a stirred, aerated bioreactor, comprising at least two steps:

the first growth step in the presence of at least one carbonaceous growth substrate in batch phase, carried out with a concentration of carbonaceous growth substrate in the range 10 to 60 g/L;

a second step for growth and enzyme production in the presence of at least one inducer carbonaceous substrate in fed batch phase in the presence of a limiting flow of carbon source in the range 50 to 140 mg per gram of cellular biomass per hour.

2. A process according to claim 1, in which the concentration of carbonaceous growth substrate is in the range 10 to 20 g/L.

3. A process according to claim 2, in which the concentration of carbonaceous substrate is in the range 12 to 17 g/L.

4. A process according to claim 1, in which the flow of the carbon source is in the range 70 to 100 mg per gram of cellular biomass per hour.

5. A process according to claim 4, in which the flow of the carbon source is in the range 80 to 90 mg per gram of cellular biomass per hour.

6. A process according to claim 1, in which the bioreactor has a volumetric oxygen transfer coefficient, k_La, in the range 40 to 180 h⁻

1.

7. A process according to claim 1, in which the bioreactor has a volumetric oxygen transfer coefficient, k_La, in the range 40 to 150 h⁻

1.

8. A process according to claim 1, in which the carbonaceous growth substrate is selected from lactose, glucose, xylose, residues obtained after ethanolic fermentation of monomeric sugars of enzymatic hydrolysates of cellulosic biomass and/or a crude extract of hydrosoluble pentoses deriving from pre-treatment of a cellulosic biomass.

9. A process according to claim 1, in which the inducer carbonaceous substrate is lactose, cellobiose, sophorose, residues obtained after ethanolic fermentation of monomeric sugars of enzymatic hydrolysates of cellulosic biomass and/or a crude extract of hydrosoluble pentoses deriving from pre-treatment of a cellulosic biomass.

10. A process according to claim 1, in which the carbonaceous growth substrate selected for producing biomass is introduced into the fermenter before sterilization.

11. A process according to claim 1, in which the carbonaceous growth substrate selected for producing the biomass is sterilized separately and introduced into the bioreactor after sterilization.

12. A process according to claim 1, in which the inducer carbonaceous substrate introduced during the fed batch phase is sterilized independently before being introduced into the reactor.

13. A process according to claim 1, in which the strain used is a strain of Trichoderma reesei.

14. A process according to claim 1, in which the strain used is a strain of Trichoderma reesei modified by genetic mutation, selection or recombination.