METHODS FOR THE ELECTROCHEMICAL TREATMENT OF SELF-ASSEMBLED MONOLAYERS

• US 20140305811A1
• Filed: 03/12/2014
• Published: 10/16/2014
• Est. Priority Date: 03/15/2013
• Status: Abandoned Application

• Current Assignee: Genmark Diagnostics Incorporated (Roche Holding AG)
• Original Assignee: Genmark Diagnostics Incorporated (Roche Holding AG)
• Inventors: Kayyem, Jon Faiz, Ford, Sean, Rusterholz, Ken, Bender, William, Argueta, Claudia C.
• Application Number:
  US14/206,871
• Publication Number:
  US 20140305811A1
• Time in Patent Office: Days
• US Class Current: 205/782
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68

Abstract

The present invention provides compositions and methods directed to an electrode initialization step for the electrochemical treatment of monolayers used in electrochemical detection of target analytes on the surface of a monolayer. Electrode initialization creates a more stable monolayer, and resolves variability within the electrochemical signal detected on the monolayer.

Claims

1. A method for detecting the presence of a target analyte in a sample, the method comprising:

a) providing an electrode comprising a monolayer and a capture binding ligand;

b) initializing the electrode;

c) hybridizing a probe to said target analyte to form an assay complex; and

d) detecting the presence or absence of said target analyte.

2. The method of claim 1, wherein the detecting provides a better signal to noise ratio than a method wherein no initializing step b) is performed.

3. The method of claim 1 wherein said target analyte is selected from the group consisting of a nucleic acid, a protein, and a combination thereof.

4. The method claim 1, wherein the monolayer is a self-assembled monolayer (SAM).

5. The method of claim 4, wherein the SAM comprises insulators.

6. The method claim 4, wherein the SAM comprises conductive oligomers.

7. The method of claim 1 wherein the capture binding ligand further comprises an attachment linker.

8. The method of claim 1 wherein the initialization step comprises applying an electronic signal to said electrode.

9. The method of claim 1 wherein said probe is attached to an electron transfer moiety (ETM).

10. The method of claim 9 wherein the ETM is responsive to an input waveform.

11. The method of claim 9 wherein the ETM is a metallocene.

12. The method of claim 11 wherein the metallocene is a ferrocene.

13. The method of claim 12 wherein the ferrocene is a ferrocene derivative.

14. The method of claim 9 wherein the probe is covalently attached to the ETM.

15. The method of claim 1 wherein the detecting comprises applying an input signal to said electrode.

16. The method of claim 15 wherein the input signal generates an output waveform based substantially on electron transfer between said ETM and said electrode.

17. The method of claim 1 wherein the electrode is gold.

18. The method of claim 15 wherein the input signal is AC/DC offset.

19. The method of claim 18 wherein the AC frequency ranges from 90-1000 Hz.

20. The method of claim 19 wherein the AC voltage ranges from −

150 to 880 mV rms.

21. The method of claim 1 wherein electrode initialization is performed for 0.5 seconds.

22. The method of claim 1 wherein electrode initialization if performed for 1 second.

23. The method of claim 1 wherein electrode initialization is performed for 2 seconds.

24. The method of claim 1 wherein electrode initialization is performed for 5 seconds.

25. The method of claim 1 wherein electrode initialization is performed for 10 seconds.

26. The method of claim 1 wherein electrode initialization is performed for longer than 10 seconds.