Assays for Analyte Homologs
First Claim
1. A method of adjusting a contribution of one of two analyte homologs to an amount of signal obtained in an assay for determining a total amount of the two analyte homologs in a sample, the method comprising:
- (a) providing in combination in an assay medium;
(i) a sample suspected of containing the two analyte homologs,(ii) reagents for conducting an assay for determining the total amount of the two analyte homologs in the sample, wherein the reagents comprises at least one assay receptor that binds to the two analyte homologs, and(iii) a non-assay receptor that has a greater binding affinity for whichever of the two analyte homologs whose contribution to the amount of signal is to be adjusted, wherein an amount of the non-assay receptor is sufficient to achieve an adjustment of the contribution of the analyte homolog to the signal; and
(b) conducting the assay for determining the total amount of the two analyte homologs.
1 Assignment
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Accused Products
Abstract
Methods include adjusting a contribution of one of two analyte homologs to an amount of signal obtained in an assay for determining a total amount of the two analyte homologs in a sample. A non-assay receptor is employed that has a greater binding affinity for whichever of the two analyte homologs whose contribution to the amount of signal is to be adjusted. An amount of the non-assay receptor is sufficient to achieve an adjustment of the contribution of the analyte homolog to the signal. The assay for determining the total amount of the two analyte homologs is conducted where the assay utilizes at least one assay antibody.
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Citations
19 Claims
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1. A method of adjusting a contribution of one of two analyte homologs to an amount of signal obtained in an assay for determining a total amount of the two analyte homologs in a sample, the method comprising:
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(a) providing in combination in an assay medium; (i) a sample suspected of containing the two analyte homologs, (ii) reagents for conducting an assay for determining the total amount of the two analyte homologs in the sample, wherein the reagents comprises at least one assay receptor that binds to the two analyte homologs, and (iii) a non-assay receptor that has a greater binding affinity for whichever of the two analyte homologs whose contribution to the amount of signal is to be adjusted, wherein an amount of the non-assay receptor is sufficient to achieve an adjustment of the contribution of the analyte homolog to the signal; and (b) conducting the assay for determining the total amount of the two analyte homologs. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of determining in a sample an amount of a first analyte homolog and a second analyte homolog, the method comprising:
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(a) providing in combination in an assay medium; (i) a sample suspected of containing the first analyte homolog and the second analyte homolog, (ii) reagents for conducting an assay for the first analyte homolog and the second analyte homolog wherein the reagents comprise an assay antibody capable of binding to each of the first analyte homolog and the second analyte homolog to form complexes and a member of a signal producing system that produces a signal in relation to the amount of the first analyte homolog and the second analyte homolog in the complexes, and (iii) a non-assay receptor that has a greater binding affinity for whichever of the first analyte homolog or the second analyte homolog whose contribution to the amount of signal is to be adjusted, wherein an amount of the non-assay receptor is sufficient to achieve an adjustment of the contribution of the first analyte homolog or the second analyte homolog to the signal; (b) incubating the assay medium under conditions for forming the complexes, (c) determining the amount of signal from the complexes, and relating the amount of signal to the amount of the first analyte homolog and the second analyte homolog in the sample. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A method of determining in a sample a total amount of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3, the method comprising:
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(a) providing in combination in an assay medium; (i) a sample suspected of containing 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3, (ii) reagents for conducting an assay for 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 wherein the reagents comprise an assay antibody capable of binding to 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 to form complexes and a member of a signal producing system that produces a signal in relation to the amount of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 complexes, and (iii) a non-assay antibody that has a greater binding affinity for 25-hydroxy vitamin D2, wherein an amount of the non-assay antibody is sufficient to achieve an adjustment of the contribution of 25-hydroxy vitamin D2 to the amount of signal; (b) incubating the assay medium under conditions for forming the complexes, (c) determining the amount of signal from the complexes, and relating the amount of signal to the total amount of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 in the sample. - View Dependent Claims (16, 17, 18, 19)
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Specification