CAPTURING OF CELL FLUID AND ANALYSIS OF ITS COMPONENTS UNDER OBSERVATION OF CELLS AND INSTRUMENTS FOR THE CELL FLUID CAPTURING AND THE ANALYSIS
First Claim
1. A method for capturing cellular components from a single cell and performing mass spectrometry on the components, said method comprising:
- inserting a nanospray ionization capillary tip into a specific region of the cell under observation, whose opening diameter is corresponding to the specific region of the cell under observation with a microscope, wherein the nanospray ionization capillary tip comprises a filament in the interior,capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip,supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip,applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, andperforming the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.
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Abstract
A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.
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Citations
24 Claims
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1. A method for capturing cellular components from a single cell and performing mass spectrometry on the components, said method comprising:
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inserting a nanospray ionization capillary tip into a specific region of the cell under observation, whose opening diameter is corresponding to the specific region of the cell under observation with a microscope, wherein the nanospray ionization capillary tip comprises a filament in the interior, capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 24)
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11. A system for capturing cellular components from a single cell and performing mass spectrometry on the components said system comprising:
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a microscope to observe the dynamic morphological change of cells which are subjected to mass spectroscopic analysis, nanospray ionization capillary to ionize the cellular components for the mass spectrometry, said nanospray ionization capillary further comprising a tip portion having an opening and a filament in the interior, for capturing the cellular components of a specific region of the cell by inserting the tip to the specific region of the cell, whose size is not bigger than the size of the specific region of the cell, and a back side portion from which ionization supporting solvent is supplied, a mass spectrometer which has a sample inlet hole to analyze the cellular components after the ionization, and a high voltage power supply which applies an electric field between the sample inlet and the nanospray ionization capillary, wherein the nanospray ionization capillary tip is configured to penetrate into the cell under observation of the microscope to capture the cell components from the specific region of the cell, the captured components are ionized by applying the electric field between the nanospray ionization capillary and the inlet hole of the mass spectrometer and wherein the system is configured to introduce the solvent into back side portion of the nanospray ionization capillary. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A method for capturing cellular components from a single cell and performing mass spectrometry on the components, said method comprising:
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inserting a nanospray ionization capillary tip into a specific region of a cell whose opening diameter is not bigger than a size of the specific region of the cell, wherein the a nanospray ionization capillary tip comprises a filament in the interior, capturing the cellular components in the specific region from the tip of the nanospray ionization capillary, supplying ionization supporting solvent from a back-end of the nanospray ionization capillary, keeping the components at the tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip whereby the cellular components are ionized and introduced to the mass spectrometer, and performing the mass spectrometry to the introduced cellular components.
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21. A system for capturing cellular components from a single cell and performing mass spectrometry on the component, said system comprising:
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a nanospray ionization capillary to ionize the cellular components for the mass spectrometry, said nanospray ionization capillary further comprising a tip portion having an opening and a filament in the interior, for capturing the cellular components of a specific region of the tissues by inserting the tip into the specific region of the tissues for capturing the cellular components whose size is not bigger than the size of the specific region of a cell, and a back side portion from which ionization supporting solvent is supplied, a mass spectrometer which has a sample inlet hole to analyze the cellular components after the ionization, a high voltage power supply which applies an electric field between the sample inlet and the nanospray ionization capillary, wherein the nanospray ionization capillary tip penetrates into the tissue to capture the cellular components, the captured components are ionized by applying the electric field between the nanospray ionization capillary and the inlet hole of the mass spectrometer, and wherein the system is configured to introduce the solvent into the back side portion of the nanospray ionization.
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22. A method for capturing biological fluids or liquids in less than micro meter region and performing mass spectrometry to thereof, said method comprising:
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inserting a nanospray ionization capillary tip into a specific region of a cell whose opening diameter is not bigger than a size of the specific region of the cell, wherein the a nanospray ionization capillary tip comprises a filament in the interior, capturing components in the specific region with the tip of the nanospray ionization capillary and keeping the components at the tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary, ionizing the components using nanospray ionization by applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, performing mass spectrometry for the introduced cellular components.
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23. A system for capturing biological fluids or liquids in less than micro meter region and performing mass spectrometry to thereof, said system comprising:
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a nanospray ionization capillary to ionize cellular components for the mass spectrometry, said nanospray ionization capillary further comprising a tip portion having an opening and a filament in the interior, for capturing the cellular components of a specific region of the biological fluids or liquids by inserting the tip into a specific region of a cell whose size is not bigger than the size of the specific region of the cell, and a back side portion from which ionization supporting solvent is supplied, a mass spectrometer which has a sample inlet hole to analyze the cellular components after the ionization, a high voltage power supply which applies an electric field between the sample inlet and the nanospray ionization capillary, wherein the nanospray ionization capillary tip penetrates into the cell to capture the cellular components, the captured components are ionized by applying the electric field between the nanospray ionization capillary and the inlet hole of the mass spectrometer, and wherein the system is configured to introduce the solvent into back side portion of the nanospray ionization capillary.
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Specification