METHOD AND DEVICE FOR SAMPLE PROCESSING
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Abstract
In accordance with an aspect of the present disclosure there is provided an apparatus for the processing of biological sample. The apparatus comprises a first sheet of material, a second sheet of material bonded to the first sheet of material, and a plurality of chambers defined between the first sheet of material and the second sheet of material, the plurality of chambers including a sample dissociation chamber including an inlet and an outlet; a waste collection chamber including an inlet in fluid communication with the outlet of the sample dissociation chamber, and a cell refinement chamber including an inlet in fluid communication with the sample dissociation chamber and an outlet.
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Citations
55 Claims
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1-35. -35. (canceled)
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36. An apparatus for processing biological samples, the apparatus comprising:
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a first sheet of material; a second sheet of a flexible material bonded to the first sheet of material; a plurality of chambers defined between the first sheet of material and the second sheet of material, the plurality of chambers including; a first chamber including an inlet configured to receive a fluid solution, a sample inlet configured to receive a biological sample, and an outlet, a second chamber configured to receive waste fluid, the second chamber including an inlet in fluid communication with the outlet of the first chamber, and a third chamber including an inlet in fluid communication with the first chamber and an outlet; a first filtration mesh disposed between the inlet and the outlet of at least one of the first chamber and the third chamber; and a first flow control device configured to control a flow of fluid between the first chamber and at least one of the second chamber and the third chamber. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43)
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44. A method of processing a sample in a tissue processing system, the method comprising:
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providing a tissue processing device including; a first sheet of material, a second sheet of a flexible material bonded to the first sheet of material, a plurality of chambers defined between the first sheet of material and the second sheet of material, the plurality of chambers including; a first chamber including an inlet and an outlet; a second chamber including an inlet in fluid communication with the outlet of the first chamber, and a third chamber including an inlet in fluid communication with the first chamber and an outlet, a first filtration mesh disposed between the inlet and the outlet of at least one of the first chamber and the third chamber, and a flow control device in fluid communication with the first chamber, the second chamber and the third chamber; introducing a sample to be processed into the first chamber through the inlet of the first chamber; introducing a fluid solution to the first chamber of the tissue processing device; washing the sample by mixing the fluid solution with the sample; and draining a waste fluid from the first chamber to the second chamber. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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Specification