METHOD AND DEVICE FOR SAMPLE PROCESSING

• US 20140315303A1
• Filed: 12/06/2012
• Published: 10/23/2014
• Est. Priority Date: 12/07/2011
• Status: Abandoned Application

• Current Assignee: Cytovera Inc.
• Original Assignee: Cytover Incorporated
• Inventors: Huang, Lotien R.
• Application Number:
  US14/363,260
• Publication Number:
  US 20140315303A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0457 passive flow or gravitation
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 15/06 Investigating concentration of particle suspensions by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10

Abstract

In accordance with an aspect of the present disclosure there is provided an apparatus for the processing of biological sample. The apparatus comprises a first sheet of material, a second sheet of material bonded to the first sheet of material, and a plurality of chambers defined between the first sheet of material and the second sheet of material, the plurality of chambers including a sample dissociation chamber including an inlet and an outlet; a waste collection chamber including an inlet in fluid communication with the outlet of the sample dissociation chamber, and a cell refinement chamber including an inlet in fluid communication with the sample dissociation chamber and an outlet.

Claims

1-35. -35. (canceled)

36. An apparatus for processing biological samples, the apparatus comprising:

a first sheet of material;

a second sheet of a flexible material bonded to the first sheet of material;

a plurality of chambers defined between the first sheet of material and the second sheet of material, the plurality of chambers including;

a first chamber including an inlet configured to receive a fluid solution, a sample inlet configured to receive a biological sample, and an outlet,

a second chamber configured to receive waste fluid, the second chamber including an inlet in fluid communication with the outlet of the first chamber, and

a third chamber including an inlet in fluid communication with the first chamber and an outlet;

a first filtration mesh disposed between the inlet and the outlet of at least one of the first chamber and the third chamber; and

a first flow control device configured to control a flow of fluid between the first chamber and at least one of the second chamber and the third chamber.

37. The apparatus of claim 36, wherein the first filtration mesh comprises pores having pore sizes of between about 20 micrometers and about 50 micrometers.

38. The apparatus of claim 36, wherein the first filtration mesh is included in the third chamber, and wherein the first chamber includes a second filtration mesh having a pore size of between about 30 micrometers and about 1 millimeter, the first filtration mesh having a pore size smaller than the pore size of the second filtration mesh.

39. The apparatus of claim 36, wherein the first flow control device comprises a stopcock.

40. The apparatus of claim 36, being sterile and providing a substantially isolated environment to the biological sample.

41. The apparatus of claim 36, further comprising a second flow control device configured to control introduction of at least one of a rinsing solution and a dissociation solution into the first chamber.

42. The apparatus of claim 36, further comprising a downstream processing apparatus in fluid communication with the outlet of the third chamber and including at least one microfluidic device configured to separate a fluid output from the third chamber into a first solution having a first concentration of one or more cells of interest and a second solution having a second concentration of the one or more cells of interest which is less than the first concentration.

43. The apparatus of claim 36, wherein the sample inlet comprises a connector configured to receive a syringe.

44. A method of processing a sample in a tissue processing system, the method comprising:

providing a tissue processing device including;

a first sheet of material,

a second sheet of a flexible material bonded to the first sheet of material,

a plurality of chambers defined between the first sheet of material and the second sheet of material, the plurality of chambers including;

a first chamber including an inlet and an outlet;

a second chamber including an inlet in fluid communication with the outlet of the first chamber, and

a third chamber including an inlet in fluid communication with the first chamber and an outlet,

a first filtration mesh disposed between the inlet and the outlet of at least one of the first chamber and the third chamber, and

a flow control device in fluid communication with the first chamber, the second chamber and the third chamber;

introducing a sample to be processed into the first chamber through the inlet of the first chamber;

introducing a fluid solution to the first chamber of the tissue processing device;

washing the sample by mixing the fluid solution with the sample; and

draining a waste fluid from the first chamber to the second chamber.

45. The method of claim 44, further comprising retrieving the washed sample from the tissue processing device.

46. The method of claim 44, further comprising

introducing a dissociation solution to the first chamber;

mixing the dissociation solution with the sample in the first chamber;

incubating the sample for a period of time at a first temperature in the first chamber, incubating the sample releasing a population of cells of interest;

transferring the population of cells of interest to the third chamber;

passing the population of cells of interest through the first filtration mesh; and

harvesting the population of cells of interest from the outlet of the third chamber.

47. The method of claim 46, wherein the period of time is between about 5 minutes and about 30 hours.

48. The method of claim 46, wherein the period of time is between about 10 minutes and about 120 minutes.

49. The method of claim 46, wherein the first temperature is about 37 degrees Celsius.

50. The method of claim 46, wherein the dissociation solution comprises an enzyme.

51. The method of claim 50, wherein the dissociation solution comprises collagenase.

52. The method of claim 44, wherein the sample comprises an adipose tissue.

53. The method of claim 46, wherein the cells of interest comprise stem cells.

54. The method of claim 46, further comprising washing, concentrating, and reducing red blood cells from the population of cells of interest by passing the population of cells of interest through a microfluidic device.

55. The population of cells of interest of the method of claim 54.