METHOD FOR EXTRACTING NUTRIENTS FROM ORGANIC MATERIALS

• US 20140322807A1
• Filed: 12/06/2012
• Published: 10/30/2014
• Est. Priority Date: 12/06/2011
• Status: Abandoned Application

• Current Assignee: Innovative Bios LLC
• Original Assignee: Innovative Bios LLC
• Inventors: Gustin, John Paul, Repac, Jason Allen
• Application Number:
  US14/360,176
• Publication Number:
  US 20140322807A1
• Time in Patent Office: Days
• US Class Current: 435/348
• CPC Class Codes: B09B 3/80 involving an extraction step
• CPC Class Codes: C12N 1/12 Unicellular algae; Culture media therefor as new plants A01H13/00
• CPC Class Codes: C12N 1/16 Yeasts; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/38 Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound C12N1/34 takes precedence
• CPC Class Codes: C12N 5/0018 Culture media for cell or tissue culture media for specific animal cell type C12N5/06

Abstract

Processes for extracting and recovering nutrients from organic wastes to create a cell culture broth for microorganisms involve the main steps of mixing, solid/liquid separation, optimization, and sterilization. In an embodiment, the method for converting organic waste material into a cell culture broth or growth media includes: (a) mixing an organic waste material with one or more solvent to create a mixture of liquids and solids under substantially turbulent conditions; (b) separating the mixture of liquids and solids into a liquid stream and solid stream; and (c) sterilizing the liquid stream, whereby the cell culture broth or growth media comprises the sterilized liquid stream.

Claims

1. A method for converting organic waste material into a cell culture broth or growth media comprising:

mixing an organic waste material with one or more solvent under substantially turbulent conditions to create a mixture of liquids and solids;

separating the mixture of liquids and solids into a liquid stream and solid stream; and

sterilizing the liquid stream,

wherein the cell culture broth or growth media comprises the sterilized liquid stream.

2. The method of claim 1, wherein the culture broth and/or growth media is suitable for the propagation, culture, fermentation, or maintenance of bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells.

3. The method of claim 1, wherein the organic waste material is selected from the group consisting of livestock manure, bedding, litter, human organic waste, plant waste and mixtures thereof.

4. The method of claim 3, wherein the organic waste material is processed.

5. The method of claim 4, wherein the organic waste material is processed by milling, composting, pyrolysis, autoclaving, gasification, anaerobic digestion, combustion, and combinations thereof.

6. The method of claim 1, wherein the organic waste material comprises plant waste free of livestock manure, bedding, litter, and human organic waste.

11. The method of claim 1 further comprising:

analyzing the organic waste material for chemical composition prior to mixing;

analyzing the liquid stream and the solid stream for chemical composition after separating the mixture of liquids and solids into a liquid stream and solid stream;

optimizing the composition of the liquid stream depending on the desired use of the cell culture broth or growth media; and

optionally selecting one or more solvent based on the chemical composition of the organic waste material prior to mixing the organic waste material with the one or more solvent.

12. The method of claim 11, wherein the step of optimizing comprises one or more of (a) adding nutrients or chemicals to the liquid stream, (b) removing and/or deactivating excess nutrients or chemicals from the liquid stream, (c) removing and/or deactivating antibiotics and other growth harming substances from the liquid stream and combinations thereof.

17. The method of claim 1, wherein the one or more solvent is water, alcohol or an organic solvent.

18. The method of claim 1, wherein the step of mixing comprises one or more of the following:

a residence time from about 15 minutes to about 3 hours;

heating organic waste material in one or more solvent;

heating up to about 100 degrees C.;

mixing under turbulent conditions;

mixing under conditions having a Reynolds number (Re) of greater than 2000;

mixing under transitional conditions;

agitation;

or

agitation for about 30 to 60 minutes at about 150 to 200 rpm.

25. The method of claim 1, wherein the step of separating the mixture of liquids and solids into a liquid stream and solid stream comprises filtration, gravitational settling, decanting, centrifugation, or combinations thereof.

26. The method of claim 1, wherein the liquid stream comprises nutrients, and one or more solvent.

27. The method of claim 1, wherein the step of sterilizing the liquid stream comprises filtration, irradiation, chemical treatment, heating, pressurization, or combinations thereof.

28. The method of claim 27, wherein the step of sterilizing the liquid stream comprises heating the liquid stream to a temperature of about 121 degrees C. at 15 psig and maintaining the temperature for at least 15 minutes.

29. The method of claim 1 further comprising anaerobic digestion of the solid stream, gasification of the solid stream, combustion of the solid stream, pyrolysis of the solid stream or combinations thereof.

30. The method of claim 1 further comprising reusing the solid stream in the process by mixing the solid stream with the organic materials and optionally anaerobic digestion of the solid stream prior to mixing with the organic materials.

31. The method of claim 1, wherein the solid stream is suitable for use as a plant fertilizer.

33. The method of claim 1 further comprising one or more of the following steps:

adding nutrients or chemicals to the liquid stream to increase the growth rate of bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells as compared to a growth rate of the bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells without the nutrients or chemicals;

removing and/or deactivating excess nutrients or chemicals from the liquid stream to increase the growth rate of bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells as compared to a growth rate of the bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells with the nutrients or chemicals;

removing and/or deactivating antibiotics and other growth harming substances from the liquid stream to increase the growth rate of bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells as compared to a growth rate of the bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells with the antibiotics and other growth harming substances;

or

the combinations thereof.

36. The method of claim 1, wherein the cell culture broth or growth media is supplemented to achieve optimal growth conditions of bacteria, algae, yeast, synthetically derived microorganisms, insect cells, plant cells, or mammalian cells.

37. A cell culture broth or growth media produced by the method of claim 1.

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