MICROFLUIDIC CARTRIDGE AND METHOD OF MAKING SAME
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Abstract
The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.
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Citations
37 Claims
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1-17. -17. (canceled)
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18. A method of isolating a plurality of polynucleotide-containing samples on a microfluidic cartridge, the method comprising:
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providing a first reaction chamber; providing a second reaction chamber; providing a first inlet port, the first inlet port in fluid communication with the first reaction chamber; providing a second inlet port, the second inlet port in fluid communication with the second reaction chamber; providing a first outlet in fluid communication with the first reaction chamber; providing a second outlet in fluid communication with the second reaction chamber; providing a first set of microfluidic valves; providing a second set of microfluidic valves; actuating the first set of microfluidic valves to isolate the first reaction chamber from the first inlet port and the first outlet; and independent of the actuation of the first set of microfluidic valves, actuating the second set of microfluidic valves to isolate the second reaction chamber from the second inlet port and the second outlet, the isolation effected by the first and the second set of microfluidic valves preventing movement of fluid into and out of the first and the second reaction chambers. - View Dependent Claims (19, 20, 21, 22)
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23. A method of isolating a plurality of polynucleotide-containing samples, the method comprising:
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providing a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another; an inlet and an outlet; a first valve and a second valve; a reaction chamber, a first channel leading from the inlet, via the first valve, to the reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to the outlet, isolating the reaction chamber in at least two of the plurality of sample lanes from the inlet and the outlet to prevent movement of fluid into or out of the reaction chamber by closing the first valve and the second valve, the first valve in at least one of the plurality of sample lanes closed independently of another first valve. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30)
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31. A method of isolating a plurality of polynucleotide-containing samples, the method comprising:
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introducing a first sample into a first inlet port, wherein the first sample flows from the first inlet port through a first valve spaced from the first inlet port into a first amplification chamber and toward a second valve spaced from the first amplification chamber; introducing a second sample into a second inlet port, wherein the second sample flows from the second inlet port through a third valve spaced from the second inlet port into a second amplification chamber and toward a fourth valve spaced from the second amplification chamber; closing the first valve and the second valve to isolate the first sample in the first amplification chamber; independent of the closing of the first valve and the second valve, closing the third valve and the fourth valve in order to isolate the second sample in the second amplification chamber independent of the isolation of the first sample in the first amplification chamber. - View Dependent Claims (32, 33, 34, 35, 36, 37)
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Specification