METHOD FOR DETECTING CELLS WITH ELASTIC CELL MEMBRANES

• US 20140326071A1
• Filed: 05/06/2013
• Published: 11/06/2014
• Est. Priority Date: 05/06/2013
• Status: Active Grant

• Current Assignee: City College of New York
• Original Assignee: City College of New York
• Inventors: Matsui, Hiroshi, De la Rica, Roberto, Shi, Menglu
• Granted Patent: US 9,151,759 B2
• Time in Patent Office: Days
• US Class Current: 73/774
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells
• CPC Class Codes: G01N 33/48735 Investigating suspensions of cells, e.g. measuring microbe concentration by chemical means C12Q1/04; colony counters C12M1/34; concentration of particle suspensions in general G01N15/06
• CPC Class Codes: G01N 33/574 for cancer

Abstract

The subject matter disclosed in this specification pertains to a method for detecting cells with elastic cell membranes. A plurality of cells are fixed to an impedimetric transducer and osmotic stress is applied. Those cells with elastic membranes, including cancer cells, undergo a volume change which is detected by the impedimetric transducer.

Claims

1. A method for detecting cells with elastic cell membranes, the method comprising the steps of:

fixing a plurality of cells to an impedimetric transducer;

applying osmotic stress to the plurality of cells;

waiting for a predetermined time to permit the plurality of cells to change in volume due to the osmotic stress;

detecting the change in volume with the impedimetric transducer and determining cells with elastic cell membranes are present in the plurality of cells.

2. The method as recited in claim 1, wherein the impedimetric transducer comprises interdigitated electrodes.

3. The method as recited in claim 2, wherein the interdigitated electrodes each comprise a digit with a width of about three to ten micrometers.

4. The method as recited in claim 2, wherein the interdigitated electrodes have a pitch of about three to ten micrometers.

5. The method as recited in claim 2, wherein the impedimetric transducer is coated with a layer of cell adsorption material.

6. The method as recited in claim 5, further comprising fixing the plurality of cells to the layer of cell adsorption material prior to the step of applying osmotic stress.

7. The method as recited in claim 6, further comprising washing unfixed cells off of the impedimetric transducer, the step of washing unfixed cells being performed after the step of permitting the plurality of cells to become fixed to the layer of cell adsorption material and prior to the step of applying osmotic stress.

8. The method as recited in claim 5, wherein the adsorption material is selected from the group consisting of polylysine and fibronectin.

9. The method as recited in claim 1, wherein the predetermined time is at least about one minute.

10. The method as recited in claim 1, wherein the predetermined time is from about one minute to about two minutes.

11. A method for detecting cells with elastic cell membranes, the method comprising the steps of:

fixing a plurality of cells to an impedimetric transducer;

applying hyposmotic stress to the plurality of cells;

waiting for a predetermined time to permit the plurality of cells to change in volume due to the hyposmotic stress;

detecting an increase in volume with the impedimetric transducer and determining cells with elastic cell membranes are present in the plurality of cells; and

displaying the degree of increase in volume to a user.

12. The method as recited in claim 11, wherein the interdigitated electrodes each comprise a digit with a width of about three to ten micrometers.

13. The method as recited in claim 11, wherein the interdigitated electrodes have a pitch of about three to ten micrometers.

14. The method as recited in claim 11, wherein the impedimetric transducer is coated with a layer of cell adsorption material.

15. The method as recited in claim 14, further comprising fixing the plurality of cells to the layer of cell adsorption material prior to the step of applying osmotic stress.

16. The method as recited in claim 15, further comprising washing unfixed cells off of the impedimetric transducer, the step of washing unfixed cells being performed after the step of permitting the plurality of cells to become fixed to the layer of cell adsorption material and prior to the step of applying osmotic stress.

17. The method as recited in claim 15, wherein the adsorption material is selected from the group consisting of polylysine and fibronectin.

18. The method as recited in claim 11, wherein the predetermined time is at least about one minute.

19. The method as recited in claim 11, wherein the predetermined time is from about one minute to about two minutes.