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MULTIPLEXED DIAGNOSTIC DETECTION APPARATUS AND METHODS

  • US 20140329301A1
  • Filed: 05/01/2014
  • Published: 11/06/2014
  • Est. Priority Date: 11/04/2011
  • Status: Active Grant
First Claim
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1. A microfluidic cartridge comprising:

  • a plurality of sample lanes, each lane comprising a microfluidic network having;

    an inlet;

    an amplification chamber in which the polynucleotides in a liquidic sample are amplified;

    a first amplification valve upstream of the amplification chamber in an initial open position prior to introduction of a sample and a first amplification gale upstream of the amplification chamber in an initial closed position prior to introduction of a sample;

    a second amplification valve downstream of the amplification chamber in an initial open position and a second amplification gate downstream of the amplification chamber in an initial closed position prior to introduction of a sample;

    a first channel leading from the inlet, via the first amplification valve and first amplification gate, to the amplification chamber;

    a plurality of detection chambers downstream of the second amplification gate;

    a first detection valve upstream of the plurality of detection chambers and a second detection valve downstream of the plurality of detection chambers, the first and second detection valves each in an initial open position prior to introduction of a sample; and

    a second channel leading from the amplification chamber, via the second amplification gate and first detection valve, to the plurality of detection chambers.

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