TRACKING AND CHARACTERIZING PARTICLES WITH HOLOGRAPHIC VIDEO MICROSCOPY
First Claim
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1. A method for characterizing a specimen, comprising:
- providing a holographic microscope;
providing a collimated laser beam to the holographic microscope;
scattering the collimated laser beam off the specimen to generate a scattered portion;
generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion;
recording the interference pattern for subsequent analysis; and
measuring at least one of a spatial location, a mechanical property, an optical property and a chemical property of the specimen.
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Abstract
In-line holography to create images of a specimen, such as one or more particles dispersed in a transparent medium. Analyzing these images with results from light scattering theory yields the particles'"'"' sizes with nanometer resolution, their refractive indexes to within one part in a thousand, and their three dimensional positions with nanometer resolution. This procedure can rapidly and directly characterize mechanical, optical and chemical properties of the specimen and its medium.
19 Citations
25 Claims
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1. A method for characterizing a specimen, comprising:
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providing a holographic microscope; providing a collimated laser beam to the holographic microscope; scattering the collimated laser beam off the specimen to generate a scattered portion; generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion; recording the interference pattern for subsequent analysis; and measuring at least one of a spatial location, a mechanical property, an optical property and a chemical property of the specimen. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. The method as defined in claim 26 wherein the steps characterization of properties comprises analyzing the particle hydrodynamic radius, radius and refractive index.
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15. A method for optically characterizing a specimen disposed in a medium, including the steps of providing a holographic microscope, providing a laser beam to the holographic microscope, scattering a collimated laser beam off the specimen in the medium to generate a scattered portion;
- generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion;
recording the interference pattern for subsequent analysis; and
measuring at least one of a spatial location of the specimen, a mechanical property of the specimen, an optical property of the specimen, a chemical property of the specimen, and a characteristic interaction between the specimen and the medium and a feature of the medium. - View Dependent Claims (16, 17, 18, 19, 20)
- generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion;
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21. A computer readable medium for processing information to characterize a specimen comprising,
a computer software module including computer software having a set of instructions for operating on non-transitory data characteristic of the specimen; -
the non-transitory data characteristic of the specimen related to an interference pattern arising from interaction of a laser beam scattered from the specimen and an unscattered portion of the laser beam; and the set of instructions further including a scattering function which upon execution provides non-transitory convergent solution data descriptive of properties of the specimen, thereby enabling characterization of at least one of a mechanical property, an optical property, and a chemical property of the specimen. - View Dependent Claims (22, 23, 24, 25)
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Specification