MODULAR POINT-OF-CARE DEVICES, SYSTEMS, AND USES THEREOF
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Accused Products
Abstract
The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.
29 Citations
108 Claims
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1-78. -78. (canceled)
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79. A method of detecting at least two analytes present at different concentrations in a small-volume biological sample, the method comprising:
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providing a cartridge carrying i) a sample collection unit containing ii) a biological sample having a volume of no more than about 500 microliters (μ
L), and iii) all reagent units and iv) all assay units required for analyzing said biological sample for the presence of said at least two analytes;positioning said cartridge effective that a fluid transfer device may transfer fluid from said biological sample; transferring at least a portion of said biological sample from said sample collection unit to an individual assay unit to provide a first sample portion and at least a second sample portion, wherein said second sample portion is in said individual assay unit and is not in fluid communication with said first sample portion; diluting, by at least about 10-fold, at least one of said first sample portion or said second sample portion effective that signals produced by assays indicative of said at least two analytes are within detectable ranges; performing a first assay for detecting the presence of a first analyte in a portion of the biological sample; performing a second assay for detecting the presence of a second analyte in a portion of the biological sample; Detecting a signal indicative of the presence of said first analyte; Detecting a signal indicative of the presence of said second analyte; whereby the presence of each of said at least two analytes is detected in said small-volume biological sample. - View Dependent Claims (80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108)
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Specification