HUMAN-DERIVED BACTERIA THAT INDUCE PROLIFERATION OR ACCUMULATION OF REGULATORY T CELLS
First Claim
Patent Images
1. A composition that induces proliferation and/or accumulation of regulatory T cells, the composition comprising:
- (a) one or more bacteria of the Clostridia class;
(b) a culture supernatant of one or more bacteria of (a);
(c) one or more physiologically active substance derived from (a) or (b);
or(d) a combination of any 2 or 3 of (a), (b) and (c).
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Accused Products
Abstract
Species of human-derived bacteria belonging to the Clostridia class have been shown to induce accumulation of regulatory T cells (Treg cells) in the colon and suppress immune functions. Pharmaceutical compositions containing these bacteria can be used to prevent and treat immune-mediated diseases such as autoimmune diseases.
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Citations
26 Claims
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1. A composition that induces proliferation and/or accumulation of regulatory T cells, the composition comprising:
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(a) one or more bacteria of the Clostridia class; (b) a culture supernatant of one or more bacteria of (a); (c) one or more physiologically active substance derived from (a) or (b);
or(d) a combination of any 2 or 3 of (a), (b) and (c). - View Dependent Claims (2, 3, 4, 5, 10, 11, 12, 13, 16, 17, 18, 19)
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6. A composition that induces proliferation and/or accumulation of regulatory T cells, the composition comprising, as an active component:
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(a) bacteria that contain DNA comprising a nucleotide sequence that has at least 90% homology with a DNA sequence designated herein as OTU136;
OTU46;
OTU221;
OTU9;
OTU296;
OTU21;
OTU166;
OTU73;
OTU174;
OTU14;
OTU55;
OTU337;
OTU314;
OTU195;
OTU306;
OTU87;
OTU86;
OTU152;
OTU253;
OTU259;
OTU281;
OTU288;
OTU334;
OTU359;
OTU362;
or OTU367; and
DNA comprising a nucleotide sequence that has a percent homology of at least 90% with DNA of one or more of the following;
Clostridium saccharogumia, Clostridium ramosum JCM1298, Clostridium ramosum, Flavonifractor plautii, Pseudoflavonifractor capillosus ATCC 29799, Clostridium hathewayi, Clostridium saccharolyticum WM1, Bacteroides sp. MANG, Clostridium saccharolyticum, Clostridium scindens, Lachnospiraceae bacterium 5—
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57 FAA, Lachnospiraceae bacterium 6—
1—
63FAA, Clostridium sp. 14616, Clostridium bolteae ATCC BAA-613, cf. Clostridium sp. MLG055, Erysipelotrichaceae bacterium 2—
2—
44A, Clostridium indolis, Anaerostipes caccae, Clostridium bolteae, Lachnospiraceae bacterium DJF_VP30, Lachnospiraceae bacterium 3—
1—
57 FAA_CT1, Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241, Ruminococcus sp. ID8, Lachnospiraceae bacterium 2—
1—
46 FAA, Clostridium lavalense, Clostridium asparagiforme DSM 15981, Clostridium symbiosum, Clostridium symbiosum WAL-14163, Eubacterium contortum, Clostridium sp. D5, Oscillospiraceae bacterium NML 061048, Oscillibacter valericigenes, Lachnospiraceae bacterium A4, Clostridium sp. 316002/08, Clostridiales bacterium 1—
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47 FAA, Blautia cocoides, or Anaerostipes caccae DSM 14662;(b) a culture supernatant of one or more bacteria of (a); (c) one or more physiologically active substance derived from (a) or (b);
or(d) a combination of any 2 or 3 of (a), (b) and (c). - View Dependent Claims (7)
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8. A composition that induces proliferation and/or accumulation of regulatory T cells, the composition comprising at least two organisms and/or substances selected from the group consisting of:
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(a) Clostridium saccharogumia, Clostridium ramosum JCM1298, Clostridium ramosum, Flavonifractor plautii, Pseudoflavonifractor capillosus ATCC 29799, Clostridium hathewayi, Clostridium saccharolyticum WM1, Bacteroides sp. MANG, Clostridium saccharolyticum, Clostridium scindens, Lachnospiraceae bacterium 5—
1—
57 FAA, Lachnospiraceae bacterium 6—
1—
63FAA, Clostridium sp. 14616, Clostridium bolteae ATCC BAA-613, cf. Clostridium sp. MLG055, Erysipelotrichaceae bacterium 2—
2—
44A, Clostridium indolis, Anaerostipes caccae, Clostridium bolteae, Lachnospiraceae bacterium DJFVP30, Lachnospiraceae bacterium 3—
1—
57 FAA_CT1, Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241, Ruminococcus sp. ID8, Lachnospiraceae bacterium 2—
1—
46 FAA, Clostridium lavalense, Clostridium asparagiforme DSM 15981, Clostridium symbiosum, Clostridium symbiosum WAL-14163, Eubacterium contortum, Clostridium sp. D5, Oscillospiraceae bacterium NML 061048, Oscillibacter valericigenes, Lachnospiraceae bacterium A4, Clostridium sp. 316002/08, Clostridiales bacterium 1—
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47 FAA, Blautia cocoides, and Anaerostipes caccae DSM 14662;(b) a culture supernatant of one or more bacteria of (a); (c) one or more physiologically active substances derived from (a) or (b);
or(d) a combination of any 2 or 3 of (a), (b) and (c).
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9. (canceled)
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14. A method of inducing proliferation, accumulation or both proliferation and accumulation of regulatory T cells in an individual in need thereof, comprising administering, to the individual, at least one organism or substance selected from the group consisting of:
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(a) at least one bacterium containing DNA comprising a nucleotide sequence having at least 97% homology with any of SEQ ID NOs. 19 to 44; (b) at least one physiologically active substance derived from bacteria containing DNA comprising a nucleotide sequence having at least 90% homology with any of SEQ ID NOs. 19 to 44; and (c) a culture supernatant of one of the following;
Clostridium saccharogumia, Clostridium ramosum JCM1298, Clostridium ramosum, Flavonifractor plautii, Pseudoflavonifractor capillosus ATCC 29799, Clostridium hathewayi, Clostridium saccharolyticum WM1, Bacteroides sp. MANG, Clostridium saccharolyticum, Clostridium scindens, Lachnospiraceae bacterium 5—
1—
57 FAA, Lachnospiraceae bacterium 6—
1—
63 FAA, Clostridium sp. 14616, Clostridium bolteae ATCC BAA-613, cf. Clostridium sp. MLG055, Erysipelotrichaceae bacterium 2—
2—
44A, Clostridium indolis, Anaerostipes caccae, Clostridium bolteae, Lachnospiraceae bacterium DJF_VP30, Lachnospiraceae bacterium 3—
1—
57 FAA_CT1, Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241, Ruminococcus sp. ID8, Lachnospiraceae bacterium 2—
1—
46 FAA, Clostridium lavalense, Clostridium asparagiforme DSM 15981, Clostridium symbiosum, Clostridium symbiosum WAL-14163, Eubacterium contortum, Clostridium sp. D5, Oscillospiraceae bacterium NML 061048, Oscillibacter valericigenes, Lachnospiraceae bacterium A4, Clostridium sp. 316002/08, Clostridiales bacterium 1—
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47 FAA, Blautia cocoides, and Anaerostipes caccae DSM 14662. - View Dependent Claims (15)
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20. A method of selectin g a bacterium that induces proliferation and/or accumulation of regulatory T cells, comprising
(a) isolating the bacterial spore-forming fraction from a fecal or biopsy sample obtained from a mammal; -
(b) plating serial dilutions of the isolated spore-forming fraction, and (c) picking a single colony from the culture plate. - View Dependent Claims (26)
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21-25. -25. (canceled)
Specification