TOUCHSCREEN DEVICE AND METHODS FOR USE IN DETECTION OF MICRORNA

• US 20140342442A1
• Filed: 05/15/2014
• Published: 11/20/2014
• Est. Priority Date: 05/15/2013
• Status: Abandoned Application

• Current Assignee: Bioo Scientific Corporation (Revvity, Inc.)
• Original Assignee: Bioo Scientific Corporation (Revvity, Inc.)
• Inventors: Toloue, Masoud M., Mueller, Michael T., Gone, Swapna
• Application Number:
  US14/278,996
• Publication Number:
  US 20140342442A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: G01N 27/226 Construction of measuring vessels; Electrodes therefor
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68
• CPC Class Codes: G06F 2203/04104 Multi-touch detection in digitiser, i.e. details about the simultaneous detection of a plurality of touching locations, e.g. multiple fingers or pen and finger
• CPC Class Codes: G06F 3/044 by capacitive means

Abstract

A method and sensor for detecting the presence of specific target nucleic acids uses a biosensor comprising an array of single stranded probe oligonucleotides, wherein one end of each probe oligonucleotide is linked to a label and the other end of each probe oligonucleotide is attached to an electrode of a solid support. The method includes determining the capacitance at one or more of the electrodes, where a change in the capacitance of the one or more electrodes indicates the presence of target nucleic acid complementary to the single stranded oligonucleotide probe.

Claims

1-8. -8. (canceled)

9. A biosensor comprising:

a substrate comprising a plurality of capacitive sensitive electrodes;

one or more single stranded probe oligonucleotides, wherein one end of each single stranded probe oligonucleotide is linked to a label and the other end of each single stranded probe oligonucleotide is coupled to the substrate proximate to one or the plurality of electrodes;

wherein the capacitance of the electrodes changes when the coupled single stranded probe oligonucleotide is removed or altered.

10. The biosensor of claim 9, wherein the substrate is a touchscreen.

11. The biosensor of claim 9, wherein the substrate is a capacitive touchscreen.

12. The biosensor of claim 9, wherein the substrate is a projected capacitive touchscreen.

13. The biosensor of claim 9, wherein the substrate comprises a glass layer formed over one or more of the electrodes, and wherein the one or more single stranded probe oligonucleotides are coupled to the glass layer.

14. The biosensor of claim 9, wherein one or more of the single stranded probe oligonucleotides are DNA oligonucleotides.

15. The biosensor of claim 9, wherein one or more of the single stranded probe oligonucleotides are capable of hybridizing with miRNA.

16. The biosensor of claim 9, wherein one or more of the single stranded probe oligonucleotides are capable of hybridizing with cDNA.

17. The biosensor of claim 9, wherein the substrate comprises glass having an electrode array formed of indium tin oxide on a surface of the glass.

18. The biosensor of claim 9, wherein one or more of the single stranded probe oligonucleotides have a magnetic bead attached to the 5′

end, wherein the magnetic bead is the label.

19. The biosensor of claim 9, wherein one or more of the single stranded probe oligonucleotides have a magnetic bead attached to the 3′

end, wherein the magnetic bead is the label.

20. The biosensor of claim 9, wherein one or more of the single stranded probe oligonucleotides are coupled to the substrate at the 3′

end.

21. The biosensor of claim 9, wherein one or more of the single stranded probe oligonucleotides are coupled to the substrate at the 5′

end.

22. The biosensor of claim 9, wherein the substrate is incorporated into a cell phone.

23-26. -26. (canceled)

27. A biosensor comprising:

a first substrate comprising one or more electrodes on a first side of the substrate and a grounding layer on a second side of the substrate, the second side being opposite to the first side;

one or more single stranded probe oligonucleotides, wherein one end of each single stranded probe oligonucleotide is linked to a label and the other end of each single stranded probe oligonucleotide is coupled to the electrodes of the first substrate;

vias connecting the one or more electrodes on the first side of the first substrate to the grounding layer on the second side of the first substrate;

wherein the capacitance of the electrodes changes when the coupled single stranded probe oligonucleotide is removed or altered.

28. The biosensor of claim 27, where the grounding layer is placed in electrical contact with a ground.

29. The biosensor of claim 27, where the grounding layer is placed in electrical contact with a system ground.

30. The biosensor of claim 27, where the first substrate is a printed circuit board.

31. The biosensor of claim 27, where the first substrate is an integrated circuit.

32. The biosensor of claim 27, where the ground of the first substrate is in electrical contact with the system ground of the second substrate.

33-34. -34. (canceled)