PROCESS FOR THE PRODUCTION OF AN ENZYMATIC COCKTAIL USING SOLID RESIDUES FROM A PROCESS FOR THE BIOCHEMICAL COVERSION OF LIGNOCELLULOSIC MATERIALS

• US 20140349373A1
• Filed: 11/27/2012
• Published: 11/27/2014
• Est. Priority Date: 12/14/2011
• Status: Active Grant

• Current Assignee: IFP Energies Nouvelles
• Original Assignee: IFP Energies Nouvelles
• Inventors: Ben Chaabane, Fadhel, Louret, Sylvain
• Granted Patent: US 9,885,027 B2
• Time in Patent Office: Days
• US Class Current: 435/209
• CPC Class Codes: C12N 1/16 Yeasts; Culture media therefor
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12N 9/2477 Hemicellulases not provided in a preceding group
• CPC Class Codes: C12P 21/00 Preparation of peptides or proteins single cell protein C12N1/00
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: C12Y 302/01074 Glucan 1,4-beta-glucosidase (3.2.1.74)
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases:

• • a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L;
  • a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.

Claims

1. A process for the production of an enzymatic cocktail with a cellulolytic microorganism, comprising two phases:

a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L;

a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.

2. The process according to claim 1, in which said solid residue is obtained after a partial enzymatic hydrolysis.

3. The process according to claim 1, in which the portion of lignin in the solid portion of said solid residue is 45% to 80% by weight.

4. The process according to claim 1, in which said solid residue is separated from the effluent of the enzymatic hydrolysis step.

5. The process according to claim 1, in which said carbonaceous inducer substrate is used as a mixture with at least one other carbonaceous substrate selected from inducer or non-inducer sugars.

6. The process according to claim 5, in which said other carbonaceous substrate is selected from lactose, glucose, cellulosic hydrolysate, hemicellulosic hydrolysate, cellobiose and xylose, used alone or as a mixture.

7. The process according to claim 1, in which the carbonaceous production substrate contains at least 5% by weight of cellulose.

8. The process according to claim 1, in which the specific flow rate at which the carbonaceous production substrate used in said production phase b) is supplied is in the range 35 to 65 mg of carbonaceous substrate per gram of microorganism per hour.

9. The process according to claim 1, in which said carbonaceous inducer substrate is separated at the end of the step for pre-treatment of a lignocellulosic material.

10. The process according to claim 1, in which said hydrolysis step is initiated with 1 to 60 mg of enzymatic cocktail per gram of cellulose in the lignocellulosic material which has undergone a pre-treatment step.

11. The process according to claim 10, in which said hydrolysis step is carried out with 10% to 40% by weight of DM.

12. The process according to claim 1, in which the cellulolytic microorganism is selected from strains of fungi belonging to the genuses Trichoderma, Aspergillus, Penicillium or Schizophyllum.

13. The process according to claim 12, in which the cellulolytic microorganism belongs to the species Trichoderma reesei.

14. The process according to claim 1, in which the carbonaceous inducer substrate comprises at least one solid residue obtained from the step for ethanolic fermentation of enzymatic hydrolysates.