NUCLEIC ACID ENRICHMENT USING CAS9
First Claim
Patent Images
1. A method comprising:
- (a) contacting a sample comprising fragmented genomic DNA with a Cas9-gRNA complex comprising a mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in said genomic DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented genomic DNA; and
(b) isolating said Cas9-fragment complex.
1 Assignment
0 Petitions
Accused Products
Abstract
A method of enriching for a fragment of a genome, as well as corresponding compositions and kits, are provided. In certain embodiments, the method comprises: (a) contacting a sample comprising fragmented DNA with a Cas9-gRNA complex comprising mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in the DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented DNA; and (b) isolating the complex. In addition, other methods and compositions for Cas9/CRISPR-mediated nucleic acid manipulation are also provided.
-
Citations
20 Claims
-
1. A method comprising:
-
(a) contacting a sample comprising fragmented genomic DNA with a Cas9-gRNA complex comprising a mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in said genomic DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented genomic DNA; and (b) isolating said Cas9-fragment complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
-
-
20. A composition comprising a mutant Cas9 protein that has inactivated nuclease activity and a plurality of Cas9-associated guide RNA that are complementary to different sites in genomic DNA.
Specification