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NUCLEIC ACID ENRICHMENT USING CAS9

  • US 20140356867A1
  • Filed: 05/29/2014
  • Published: 12/04/2014
  • Est. Priority Date: 05/29/2013
  • Status: Active Grant
First Claim
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1. A method comprising:

  • (a) contacting a sample comprising fragmented genomic DNA with a Cas9-gRNA complex comprising a mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in said genomic DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented genomic DNA; and

    (b) isolating said Cas9-fragment complex.

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