DERIVATION OF EMBRYONIC STEM CELLS AND EMBRYO-DERIVED CELLS
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Accused Products
Abstract
This present invention provides novel methods for deriving embryonic stem cells and embryo-derived cells from an embryo without requiring destruction of the embryo. The invention further provides cells and cell lines derived without embryo destruction, and the use of the cells for therapeutic and research purposes. It also relates to novel methods of establishing and storing an autologous stem cell line prior to implantation of an embryo, e.g., in conjunction with reproductive therapies such as IVF.
6 Citations
139 Claims
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1-121. -121. (canceled)
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122. An in vitro method of producing embryonic stem (ES) cells from a blastomere, comprising:
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(a) providing a mammalian blastomere; (b) culturing the blastomere in the presence of pluripotent cells, extracellular matrix or both until said blastomere gives rise to a cluster of progeny cells; and (c) transferring the cluster of progeny cells to ES cell medium and culturing the cluster of progeny cells therein to form a culture of mammalian ES cells from the cluster of progeny cells. - View Dependent Claims (123, 124, 125, 126, 127, 128, 129, 138)
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130. A mammalian embryonic stem (ES) cell line produced from a single blastomere, by a process comprising:
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(a) providing a mammalian blastomere; (b) culturing the blastomere in the presence of pluripotent cells, extracellular matrix or both until said blastomere gives rise to a cluster of progeny cells; and (c) transferring the cluster of progeny cells to ES cell medium and culturing the cluster of progeny cells therein to form a culture of mammalian ES cells from the cluster of progeny cells. - View Dependent Claims (131, 132, 133, 134, 135, 136, 137)
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139. A human embryonic stem cell line produced from a single blastomere.
Specification