SINGLE SHOT HIGH RESOLUTION CONJUNCTIVAL SMALL VESSEL PERFUSION METHOD FOR EVALUATING MICROVASCULATURE IN SYSTEMIC AND OCULAR VASCULAR DISEASES
First Claim
1. A method for generating a small vessel perfusion map of an ocular conjunctiva, the method comprising:
- obtaining a single raw image of at least a portion of microvasculature of the ocular conjunctiva, the raw image including at least a red color channel, a blue color channel, and a green color channel;
removing the red color channel and the blue color channel from the raw image to create a color-adjusted image of the microvasculature; and
inverting the color-adjusted image to create a grayscale inverted image of the microvasculature.
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Abstract
A system and method for generating high-resolution, small vessel perfusion maps (nCPMs) of the ocular conjunctival microvasculature. Unlike current systems and methods, the present invention allows for the generation of nCPMs using only a single raw image obtained in a single image acquisition step. The method includes obtaining a single raw image of at least a portion of the microvasculature of the ocular conjunctiva. The raw image may be obtained using a digital camera and slit lamp. The red and blue color channels are removed from the raw image to create a color-adjusted image in which contrast between the microvasculature and the sclera is enhanced. The color-adjusted image is then inverted and saved as a grayscale inverted image. The grayscale inverted image may be skeletonized. The grayscale image and/or the skeletonized image may be quantitatively analyzed.
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Citations
20 Claims
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1. A method for generating a small vessel perfusion map of an ocular conjunctiva, the method comprising:
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obtaining a single raw image of at least a portion of microvasculature of the ocular conjunctiva, the raw image including at least a red color channel, a blue color channel, and a green color channel; removing the red color channel and the blue color channel from the raw image to create a color-adjusted image of the microvasculature; and inverting the color-adjusted image to create a grayscale inverted image of the microvasculature. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of evaluating microvasculature of an eye, the method comprising:
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obtaining a single raw image of at least a portion of microvasculature of the ocular conjunctiva, the image including at least a red color channel, a blue color channel, and a green color channel; removing the red color channel and the blue color channel from the raw image to create a color-adjusted image of the microvasculature; inverting the color-adjusted image to create a grayscale inverted image of the microvasculature; and skeletonizing the grayscale inverted image of the microvasculature to create a skeletonized image of the microvasculature, the microvasculature including a plurality of vessels, and skeletonizing the grayscale inverted image including digitally reducing one or more of the plurality of vessels to one or more linear shapes, each linear shape having a uniform diameter.
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17. A system for generating a small vessel perfusion map of an ocular conjunctiva, the system comprising:
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a slit lamp; and a computer in electrical communication with the slit lamp, the computer including one or more processors programmed to; obtain a single raw image of at least a portion of microvasculature of the ocular conjunctiva from the slit lamp, the raw image including at least a red color channel, a blue color channel, and a green color channel; remove the red color channel and the blue color channel to create a grayscale image of the microvasculature; invert the grayscale image; and calculate at least one conjunctival microvasculature characteristic. - View Dependent Claims (18, 19, 20)
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Specification