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METHODS AND KITS FOR DETECTION OF METHYLATION STATUS

  • US 20140363815A1
  • Filed: 12/13/2012
  • Published: 12/11/2014
  • Est. Priority Date: 12/13/2011
  • Status: Active Grant
First Claim
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1. A process for detecting 5-methylated and/or other modified cytosine residues in a nucleic acid sample comprising:

  • a) replicating said nucleic acid sample under conditions such that 5-methylated cytosine residues are maintained and said other modified cytosine residues are diluted;

    b) treating said replicated nucleic acid sample to convert unmodified cytosine residues to a uracil or thymidine residues; and

    c) reading the sequence of said replicated nucleic acid sample wherein 5-hydroxymethylated cytosine residues are identified as residues that are read by sequencing as a thymidine or uracil residue in said replicated nucleic acid sample.

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