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METHODS FOR GENERATING TRANSGENIC PLANTS

  • US 20140366223A1
  • Filed: 06/06/2014
  • Published: 12/11/2014
  • Est. Priority Date: 06/11/2013
  • Status: Active Grant
First Claim
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1. A transformation method for making transgenic plant cells, comprising:

  • a. providing a first transcriptional cassette, wherein the first transcription cassette comprises a selectable marker;

    b. providing a second transcriptional cassette comprising at least a DNA fragment of a gene which is essential for the plant cell to survive the transformation and regeneration process, wherein said fragment produces an RNA molecule;

    c. providing at least one transcriptional cassette comprising a trait gene;

    d. transforming said cassettes into a plurality of plant cells to produce a plurality of transgenic plant cells;

    e. selecting for transgenic plant cells in the presence of a selection agent using the selectable marker of step (a); and

    f. growing transgenic tissue from said transgenic plant cells of step (e),wherein transgenic plant cells with two or more copies of the transcriptional cassette of step (b) have reduced survivability through the transformation and regeneration process, and an increased percentage of said transgenic cells carrying only one copy of each transcriptional cassette is generated compared to methods without step (b).

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