METHODS FOR TREATING A SUSPENSION OF MONONUCLEAR CELLS TO FACIILTATE EXTRACORPOREAL PHOTOPHERESIS
First Claim
1. A method of extracorporeal photopheresis of mononuclear cells comprising:
- collecting a suspension of mononuclear cells containing red blood cells;
adding a lysing agent to the suspension;
incubating the suspension to lyse the red blood cells;
removing from the suspension hemoglobin freed by the lysing of the red blood cells;
adding an ultraviolet light activated substance to the suspension; and
irradiating the suspension with ultraviolet light.
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Accused Products
Abstract
A method for performing a photopheresis procedure is provided comprising collecting MNCs in a suspension comprising RBCs and plasma and lysing the red blood cells in the solution, preferably by combining the suspension with a solution to cause lysis. In one example, the solution for causing lysis of the red blood cells comprises ammonium chloride, and the suspension including the ammonium chloride is incubated to cause lysing. After lysing, the suspension may be washed to remove plasma and hemoglobin freed by the lysis of the red blood cells, and an ultraviolet light activated substance is added to the suspension. The suspension is then irradiated with ultraviolet light.
10 Citations
20 Claims
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1. A method of extracorporeal photopheresis of mononuclear cells comprising:
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collecting a suspension of mononuclear cells containing red blood cells; adding a lysing agent to the suspension; incubating the suspension to lyse the red blood cells; removing from the suspension hemoglobin freed by the lysing of the red blood cells; adding an ultraviolet light activated substance to the suspension; and irradiating the suspension with ultraviolet light. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15)
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2. (canceled)
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10. (canceled)
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16. A method for performing extracorporeal photopheresis of mononuclear cells comprising:
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collecting mononuclear cells in a suspension comprising red blood cells and plasma; combining the suspension with a lysing solution; incubating the suspension to lyse the red blood cells; removing from the suspension plasma and hemoglobin freed by the lysis of the red blood cells; adding an ultraviolet light activated agent to the suspension; and irradiating the suspension with ultraviolet light. - View Dependent Claims (17, 18)
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19. A method of extracorporeal photopheresis of mononuclear cells in a suspension of concentrated mononuclear cells that includes red blood cells and plasma, comprising:
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removing sufficient red blood cells from the suspension to substantially decrease absorption of ultraviolet light by non-target substances in the suspension; adding an ultraviolet light activated substance to the suspension; and irradiating the suspension with ultraviolet light. - View Dependent Claims (20)
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Specification