Method and System Implementing Spatially Modulated Excitation or Emission for Particle Characterization with Enhanced Sensitivity
First Claim
1. A method for characterizing particles, the method comprising:
- generating a spatially modulated excitation region;
creating relative movement between a particle and the excitation region, the particle being excited upon exposure to the excitation region to obtain a time modulated signal; and
,recording the modulated signal.
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Abstract
A method and system for using spatially modulated excitation/emission and relative movement between a particle (cell, molecule, aerosol, . . . ) and an excitation/emission pattern are provided. In at least one form, an interference pattern of the excitation light with submicron periodicity perpendicular to the particle flow is used. As the particle moves along the pattern, emission is modulated according to the speed of the particle and the periodicity of the stripe pattern. A single detector, which records the emission over a couple of stripes, can be used. The signal is recorded with a fast detector read-out in order to capture the “blinking” of the particles while they are moving through the excitation pattern. This concept enables light detection with high signal-to-noise ratio and high spatial resolution without the need of expensive and bulky optics.
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Citations
30 Claims
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1. A method for characterizing particles, the method comprising:
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generating a spatially modulated excitation region; creating relative movement between a particle and the excitation region, the particle being excited upon exposure to the excitation region to obtain a time modulated signal; and
,recording the modulated signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for characterizing particles comprising;
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moving a particle within a channel; providing an environment along the channel which causes the particle to create a time modulated signal; and
,detecting and evaluating the time modulated signal. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 25)
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24. A system for characterizing particles comprising:
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means for generating a spatially modulated excitation region; means for providing relative movement between a particle and the region, the particle being excited upon exposure to the excitation area to obtain a time modulated signal; means for recording the modulated signal.
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26. A system for characterizing particles comprising;
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a channel; a means for moving a particle within the channel; an environment along the channel operative to cause the particle to create time modulated signal; and
,a detection system to record and evaluate the time modulated signal. - View Dependent Claims (27, 28, 29, 30)
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Specification