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MODIFIED SAK GENE FOR THE PRODUCTION OF RECOMBINANT PROTEINS

  • US 20150010944A1
  • Filed: 08/28/2014
  • Published: 01/08/2015
  • Est. Priority Date: 06/04/2010
  • Status: Active Grant
First Claim
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1. A process for the preparation of heterologous protein of interest in E. coli comprising the steps of:

  • a) preparing a fusion DNA comprising a first DNA encoding modified SAK protein and a second DNA fused in the frame encoding the heterologous protein of interest,b) cloning of the fusion DNA of step a into an expression vector,c) expressing the fusion protein in E. coli cells,d) optionally refolding and purifying the fusion protein;

    e) enterokinase cleavage of fusion protein, andf) isolating and purifying the protein of interest to obtain pure heterologous protein.

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