Absolute PCR Quantification
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Abstract
The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (PCR) on said volumes, detecting PCR amplification products, analyzing said detected PCR amplification products, performing absolute quantification of the PCR target and presenting said quantification results.
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Citations
74 Claims
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1-57. -57. (canceled)
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58. A method of sampling a target nucleic acid, comprising:
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a) providing a sample tray comprising a plurality of isolated reaction zones, wherein each isolated reaction zone defines a specific reaction sub-volume, wherein the reaction sub-volumes of said different isolated reaction zones are variable reaction sub-volumes; b) exposing the sample tray to a sample comprising one or more target nucleic acid molecules under conditions wherein said sample fills said plurality of isolated reaction zones; c) detecting the target nucleic acid in said isolated reaction zones. - View Dependent Claims (59, 60, 64, 66, 67, 68, 69, 70, 71, 72, 73, 74)
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61-63. -63. (canceled)
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65. (canceled)
Specification