METHOD TO ASSIST GEL ANALYSIS AND PROCESSING AND APPARATUS FOR THE SAME

• US 20150021496A1
• Filed: 07/17/2013
• Published: 01/22/2015
• Est. Priority Date: 07/17/2013
• Status: Abandoned Application

• Current Assignee: Muhammad Imran Shabbir
• Original Assignee: Muhammad Imran Shabbir
• Inventors: Shabbir, Muhammad Imran
• Application Number:
  US13/944,043
• Publication Number:
  US 20150021496A1
• Time in Patent Office: Days
• US Class Current: 250/459.1
• CPC Class Codes: G01N 2021/6495 Miscellaneous methods
• CPC Class Codes: G01N 2021/6497 Miscellaneous applications
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44726 using specific dyes, markers or binding molecules

Abstract

A method to assist gel analysis and processing and apparatus for the same is disclosed. The method employs a tracing/marking/measuring/analyzing and referencing template of transparent sheet material comprising of a pattern made of vertical and horizontal lines that form quadrilateral shapes upon intersection. The vertical and horizontal lines are clearly visible in white light and ultraviolet light. The template sheet(s) can be inserted in a square shaped template holding assembly (70) which is used in between a UV trans-illuminator (56) and a macromolecule resolving gel matrix (54) such as agarose or polyacrylamide gels. The template pattern can be made on one sheet of transparent material or separate sheets may contain vertical and horizontal lines and the finalized pattern can be achieved by inserting all the separate sheets in the template holding assembly. Under the UV light from UV trans-illuminator by adjusting the sheets containing vertical lines (13) according to the gel matrix'"'"'s lanes (32d, 32e, 32f) and horizontal lines (17) according to the area of interest for further analysis and processing, the adjusted pattern of lines (13) and (17) can be used as a reference even in the absence of UV light.

Claims

1. A tracing/marking/measuring/analyzing and referencing template apparatus comprising:

A. A template sheet of transparent flexible plastic material of substantially square shape comprising;

(a) a pattern of at least a pair or plurality of pairs of mutually parallel vertical lines; and

(b) a pattern of at least one or more mutually parallel horizontal lines;

(c) wherein the intersection of at least one pair (or plurality of pairs) of vertical lines and at least two horizontal lines make quadrilateral shape(s); and

B. A rigid template holding assembly of substantially square shape comprising;

(a) a top UV transmittable transparent panel; and

(b) a bottom UV transmittable transparent panel; and

(c) a right panel and a left panel that are integrally attached to a back panel and a front panel/entry section and integrally attached to these four said panels are the perimeter edges of said top UV transmittable transparent panel and the bottom UV transmittable transparent panel; and

(d) the front panel/entry section having one or more shelf slots for inserting template sheet(s); and

2. The invention of claim 1, wherein the vertical lines are mutually parallel to each other and the horizontal lines are mutually parallel to each other.

3. The invention of claim 1, wherein each pair of vertical lines starting from the first line represents the respective lane of the gel matrix and each said pair of vertical lines may have same, different or any other suitable color scheme and a lane number may also be marked in between the two vertical lines up till next all respective lanes.

4. The invention of claim 1, wherein the vertical and horizontal lines are clearly viewable in ultraviolet and visible light spectrum.

5. The invention of claim 1, wherein the vertical and horizontal lines can be marked on one or more than one transparent sheets or vertical lines can be marked on one transparent sheet and each horizontal line(s) can be marked on other sheet(s).

6. The invention of claim 1, wherein the vertical and horizontal lines can be marked on transparent sheet(s) by printing, drawing or any other suitable means.

7. The invention of claim 1, wherein the template holding assembly is either permanently attached to the UV transilluminator or separate and independent entity which can be applied and removed from UV transilluminator after use and can be stored elsewhere.

8. The invention of claim 1, wherein any pattern of either vertical or horizontal lines can be permanently marked on the template holding assembly and other patterns can be marked on template sheets to be used with template holding assembly.

9. A method to assist gel analysis and processing comprising the steps of:

(a) Placing the template holding assembly on UV transilluminator;

(b) Inserting the first template sheet having pattern of pairs of vertical lines in the first shelf slot of front panel/entry section of the template holding assembly;

(c) Inserting the second template sheet having pattern of first single horizontal line in the second shelf slot of front panel/entry section of the template holding assembly;

(d) Inserting the third template sheet having pattern of second single horizontal lines in the third shelf slot of front panel/entry section of the template holding assembly;

(e) Placing the gel matrix after electrophoresis on the top UV transmittable transparent panel of the template holding assembly in line with the pattern of the first template sheet with pairs of vertical lines such that each pair of vertical lines represent/mark/trace the respective lane of the gel matrix;

(f) Observing the combined pattern of the above said three template sheets in the template holding assembly which will be visible through the gel matrix without UV light;

(g) Turning the UV transilluminator “

ON”

to emit UV light making the resolved macromolecule in the gel matrix lanes to fluoresce making its location visible in the gel matrix as well as making the combined pattern of vertical and horizontal lines to fluoresce under UV light;

(h) Changing the setting of the second template sheet having pattern of first single horizontal line by moving inward or outward in accordance with setting up the upper boundary of the area of gel matrix required to be analyzed or processed;

(i) Changing the setting of the third template sheet having pattern of second single horizontal line by moving inward or outward in accordance with setting up the lower boundary of the area of gel matrix required to be analyzed or processed;

(j) Observing the quadrilateral shapes formed by intersection of each pair of vertical lines with first single horizontal line and second single horizontal line in the final settings visible through the gel matrix represents the areas of analysis in each lane.

(k) Turning the UV transilluminator “

OFF”

to stop the emission of UV light.

(l) Processing the said quadrilateral shapes corresponding to each lane, for example gel cutting by any suitable and convenient method known in the art.

10. The method of claim 10, wherein the first template sheet comprising pairs of vertical lines to represent lanes of gel matrix can be designed according to the comb used for making the wells in the gel matrix.