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CULTURE MEDIUM OF ADIPOSE-DERIVED STEM CELL, METHOD FOR PREPARING THE SAME, AND COMPOSITION INCLUDING THE SAME FOR PROMOTING HAIR GROWTH

  • US 20150037435A1
  • Filed: 08/29/2012
  • Published: 02/05/2015
  • Est. Priority Date: 03/09/2012
  • Status: Active Grant
First Claim
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1. A method for preparing a culture medium of an adipose-derived stem cell (ADSC-T), the method comprising:

  • (a) culturing the adipose-derived stem cell after isolating the adipose-derived stem cell through a centrifuge after treating an enzyme, collagenase, to a suctioned adipose tissue;

    (b) preparing the adipose-derived stem cell (ADSC-T) expressing a T antigen of SV40 by transfecting a plasmid expression vector (pEF321β

    -T) into the adipose-derived stem cell cultured in the above step (a); and

    (c) obtaining a culture medium by culturing the adipose-derived stem cell (ADSC-T) prepared as described above.

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