Solid State Fermentation Systems and Process for Producing High-Quality Protein Concentrate and Lipids
First Claim
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1. A method of producing a non-animal based protein concentrate comprising:
- inoculating a substantially dry substrate selected from the group consisting of cereal grains, bran, sawdust, peat, oil-seed materials, wood chips, and combinations thereof;
subjecting the inoculated substrate to solid state fermentation (SSF) with a microbe selected from the group consisting of Aureobasidium pullulans, Fusarium venenatum, Sclerotium glucanicum, Sphingomonas paucimobilis, Ralstonia eutropha, Rhodospirillum rubrum, Issatchenkia spp, Aspergillus spp, Kluyveromyces and Pichia spp, Trichoderma reesei, Pleurotus ostreatus, Rhizopus spp, and combinations thereof;
incubating the inoculated substrate at a pH of less than 2 to about 3 or at a pH of greater than about 8; and
recovering the resulting proteins and microbes.
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Abstract
The present invention describes a bio-based process to produce high quality protein concentrate (HQPC) and lipids by converting plant derived materials into bioavailable protein and lipids via solid state fermentation (SSF) and hybrid-SSF, including the use of such HQPC and lipids so produced as nutrients, including use as a fish meal replacement in aquaculture diets. Also disclosed is a SSF reactor and method of using the reactor.
38 Citations
20 Claims
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1. A method of producing a non-animal based protein concentrate comprising:
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inoculating a substantially dry substrate selected from the group consisting of cereal grains, bran, sawdust, peat, oil-seed materials, wood chips, and combinations thereof; subjecting the inoculated substrate to solid state fermentation (SSF) with a microbe selected from the group consisting of Aureobasidium pullulans, Fusarium venenatum, Sclerotium glucanicum, Sphingomonas paucimobilis, Ralstonia eutropha, Rhodospirillum rubrum, Issatchenkia spp, Aspergillus spp, Kluyveromyces and Pichia spp, Trichoderma reesei, Pleurotus ostreatus, Rhizopus spp, and combinations thereof; incubating the inoculated substrate at a pH of less than 2 to about 3 or at a pH of greater than about 8; and recovering the resulting proteins and microbes. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of producing a non-animal based protein concentrate comprising:
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a) forming a feedstock and transferring feedstock to a first bioreactor; b) inoculating the feedstock with at least one microbe in an aqueous medium, wherein said microbe converts released sugars into proteins and exopolysaccharides and optionally releases enzymes into the bulk fluid; c) mixing the liquid in step (b) with an acid and optionally one or more antimicrobials; d) mixing additional solids to the mixture of step (c) to reduce the moisture level of the mixture of step (c) to about 40 to about 60% and transferring said reduced moisture mixture to a second bioreactor, wherein the mixture of step (d) is incubated in said second bioreactor for a sufficient time to convert the solids into said protein concentrate. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method of producing polyunsaturated fatty acid (PUFA) comprising:
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inoculating a substrate containing low PUFA lipids either as provided or by addition, wherein the substrate is selected from the group consisting of cereal grains, bran, sawdust, peat, oil-seed materials, wood chips, syrup, and combinations thereof; subjecting the inoculated substrate to solid state fermentation (SSF) with a microbe selected from the group consisting of Pythium, Thraustochytrium and Schizochytrium, and combinations thereof; incubating the inoculated substrate. - View Dependent Claims (19, 20)
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Specification