×

MEANS AND METHODS FOR THE DETERMINATION OF (D)-2-HYDROXYGLUTARATE (D2HG) OR (D)-2-HYDROXYADIPIC ACID

  • US 20150044716A1
  • Filed: 03/01/2013
  • Published: 02/12/2015
  • Est. Priority Date: 03/01/2012
  • Status: Active Grant
First Claim
Patent Images

1. A method for detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid in a sample, the method comprising:

  • a) contacting a sample with a reagent mixture, wherein said reagent mixture comprises;

    (i) a solvent,(ii) a dye having an oxidized state and a reduced state, wherein the reduced state can be distinguished from the oxidized state and wherein the dye is initially present in the oxidized state,(iii) an electron transfer agent,(iv) a (D)-2-hydroxyglutarate dehydrogenase enzyme, and(v) a cofactor; and

    b) detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid by measuring the production of the reduced state of the dye.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×