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Next Generation Genomic Sequencing Methods

  • US 20150056609A1
  • Filed: 10/29/2014
  • Published: 02/26/2015
  • Est. Priority Date: 05/09/2012
  • Status: Active Grant
First Claim
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1. A rapid and sensitive method of identifying a nucleic acid sequence motif of an organism comprising:

  • providing multiple nucleic acid samples wherein each sample is obtained from a different strain or serotype of the organism;

    amplifying sequences of the multiple nucleic acid samples by PCR;

    obtaining sequence information of the amplified sequences by next generation sequencing;

    determining a polymorphism in the genome of at least one strain or serotype from the sequence information obtained; and

    correlating the polymorphism identified with a phenotype or genome location of the at least one strain or serotype to identify the motif.

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