MICROFLUIDIC VALVE AND METHOD OF MAKING SAME
1 Assignment
0 Petitions
Accused Products
Abstract
The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.
66 Citations
38 Claims
-
1-18. -18. (canceled)
-
19. A method of making a microfluidic valve in a microfluidic device comprising a reactor and a window disposed relative to the reactor to allow detection of fluorescence from the reactor, the method of making comprising:
-
directing a dispensing head over an inlet hole in a microfluidic substrate; propelling a quantity of thermally responsive substance as a succession of dots from the dispensing head into the inlet hole; and maintaining a temperature of the microfluidic substrate so that the thermally responsive substance flows by capillary action from the inlet hole into a microfluidic loading channel in communication with the inlet, the microfluidic loading channel comprising a first end and a second end, the second end of the microfluidic loading channel intersecting a microfluidic flow channel at a T-junction such that the flow of thermally responsive substance stops when the thermally responsive substance reaches the microfluidic flow channel without entering the microfluidic flow channel. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
-
-
33. A method of making a microfluidic valve, the method of comprising:
-
directing a dispensing head over an inlet hole in a microfluidic substrate; propelling a quantity of thermally responsive substance from the dispensing head into the inlet hole; flowing by capillary action the thermally responsive substance from the inlet hole into a microfluidic loading channel in communication with the inlet hole, the microfluidic loading channel comprising a first end and a second end, the second end of the microfluidic loading channel intersecting a microfluidic flow channel; and stopping the flow of thermally responsive substance when the thermally responsive substance reaches the microfluidic flow channel without entering the microfluidic flow channel. - View Dependent Claims (34, 35)
-
-
36. A method of isolating a plurality of polynucleotide-containing samples on a microfluidic cartridge, the method comprising:
-
introducing a first sample into a first lane of the microfluidic cartridge, wherein the first sample flows into a first reaction chamber; introducing a second sample into a second lane of the microfluidic cartridge, wherein the second sample flows into a second reaction chamber; selectively applying heat to at least one valve in the first lane to isolate the first sample in the first reaction chamber; selectively applying heat to at least one valve in the second lane to isolate the second sample in the second reaction chamber, heat being selectively applied to the at least one valve in the second lane independent of heat being selectively applied to the at least one valve in the first lane. - View Dependent Claims (37, 38)
-
Specification