METHODS OF BEAD MANIPULATION AND FORMING BEAD ARRAYS
First Claim
1. A method of depositing beads into an array substrate, the method comprising:
- contacting a plurality of beads with a solution comprising poly ethylene glycol to form a bead mixture; and
depositing the bead mixture on a surface of the array substrate, the surface defining receptacles including a plurality of channels, microwells, or posts.
1 Assignment
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Accused Products
Abstract
According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
4 Citations
20 Claims
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1. A method of depositing beads into an array substrate, the method comprising:
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contacting a plurality of beads with a solution comprising poly ethylene glycol to form a bead mixture; and depositing the bead mixture on a surface of the array substrate, the surface defining receptacles including a plurality of channels, microwells, or posts. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of depositing beads into an array substrate, the method comprising:
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contacting a plurality of polynucleotide beads with a solution comprising poly ethylene glycol to form a bead mixture; centrifuging the bead mixture on a surface of the array substrate, the surface defining receptacles including a plurality of channels, microwells, or posts; and removing the solution from the array substrate. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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Specification