Method and Apparatus for Improved Quantitation by Mass Spectrometry
First Claim
1. A method of quantifying one or more analytes from mass spectral data, comprising:
- obtaining a first set of mass spectral data from a first set of analytes eluted from a chromatography column;
obtaining a second set of mass spectral data from a second set of analytes eluted from a chromatography column;
wherein a majority of the analytes in each data set are common to both data sets;
determining apparent abundances of at least some analytes in each data set;
selecting an analyte as a target analyte and determining for that analyte a plurality of localised neighboring analytes by their locality to the target analyte with respect to retention time;
determining a locally corrected abundance of the target analyte based on differences between the first and second data sets in the apparent abundance of the determined localised neighboring analytes; and
quantifying the target analyte based on its corrected abundance.
1 Assignment
0 Petitions
Accused Products
Abstract
A method of quantifying analytes from mass spectral data, comprising: obtaining a first set of mass spectral data from a first set of analytes eluted from a chromatography column; obtaining a second set of mass spectral data from a second set of analytes eluted from a chromatography column; determining apparent abundances of analytes in each data set; selecting a target analyte and determining localised neighboring analytes by their locality to the target analyte with respect to retention time; determining a locally corrected abundance of the target analyte based on differences between the first and second data sets in the apparent abundance of the localised neighboring analytes; and quantifying the target analyte based on its corrected abundance. A majority of the neighboring analytes are typically substantially unchanged in actual abundance between the first and second data sets and can be used for aligning the abundances between the data sets.
5 Citations
22 Claims
-
1. A method of quantifying one or more analytes from mass spectral data, comprising:
-
obtaining a first set of mass spectral data from a first set of analytes eluted from a chromatography column; obtaining a second set of mass spectral data from a second set of analytes eluted from a chromatography column;
wherein a majority of the analytes in each data set are common to both data sets;determining apparent abundances of at least some analytes in each data set; selecting an analyte as a target analyte and determining for that analyte a plurality of localised neighboring analytes by their locality to the target analyte with respect to retention time; determining a locally corrected abundance of the target analyte based on differences between the first and second data sets in the apparent abundance of the determined localised neighboring analytes; and quantifying the target analyte based on its corrected abundance. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
-
Specification