MAGNETICALLY ASSISTED PROCESSING OF A MEDIUM

• US 20150093750A1
• Filed: 04/23/2013
• Published: 04/02/2015
• Est. Priority Date: 05/16/2012
• Status: Abandoned Application

• Current Assignee: Koninklijke Philips N.V.
• Original Assignee: Koninklijke Philips N.V.
• Inventors: Ovsyanko, Mikhail Mikhaylovich, Van Der Zaag, Pieter Jan, Wimberger-Friedl, Reinhold, Feitsma, Harma Martine, Lring, Theodorus Antonius Johannes, Van Zelst, Martinus Johannes
• Application Number:
  US14/396,391
• Publication Number:
  US 20150093750A1
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: B01F 2101/23 Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
• CPC Class Codes: B01F 33/452 using independent floating stirring elements
• CPC Class Codes: C07H 1/06 Separation; Purification
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: G01N 2446/00 Magnetic particle immunoreagent carriers
• CPC Class Codes: G01N 27/745 for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098
• CPC Class Codes: G01N 33/54326 Magnetic particles

Abstract

The invention relates to a processing device (100) and a method for processing a medium in a processing chamber (121). The processing comprises the addition of magnetic particles (M) to the medium and the mixing of the medium by manipulating said magnetic particles with a time-variable magnetic field (B), particularly a partially oscillating or rotating field. The magnetic field (B) may be generated with a multipole magnetic field generator (110) comprising four subunits (111A,111B), each having a core (113A,113B) with a surrounding coil (112A,112B) and with a top surface (114A,114B), wherein all top surfaces of said subunits are preferably arranged in the same plane and wherein all cores are substantially parallel to each other.

Claims

1. A method for processing a medium comprising target particles, said method comprising the following steps:

a) providing a cartridge having a processing chamber;

b) providing a multipole magnetic field generator having at least four subunits, each subunit having a core with a top surface and a coil surrounding said core, wherein the top surfaces of all subunits are arranged adjacent to the cartridge;

c) filling the processing chamber with the medium comprising the target particles, wherein the filling is done before, during, and/or after adding magnetic particles (M) to the medium;

d) controlling the multipole magnetic field generator such that a time-variable magnetic field is generated that manipulates the magnetic particles and thus mixes the medium;

e) binding target particles of the medium to the magnetic particles;

f) controlling the multipole magnetic field generator such that the magnetic particles are attracted to a surface of the processing chamber and removing the remaining medium from the processing chamber.

10. The method according to claim 1,

characterized in that a new medium is introduced into the processing chamber after step f).

11. The method according to claim 10,

characterized in that target components bound to magnetic particles are released into the new medium.

12. The method according to claim 11,

characterized in that a time-variable magnetic field is generated that manipulates the magnetic particles and thus mixes the medium during the release of the target components into the new medium.

13. The method according to claim 12,

characterized in that an additional magnetic field generator is arranged opposite to the multipole magnetic field generator with respect to the processing chamber and activated during the release of the target components into the new medium.

15. The method according to claim 1,

characterized in that the medium in step c) comprises a polyalkylene glycol, particularly in an amount ranging between about 1 wt-% and about 20 wt-%.

2. A processing device for processing a medium comprising target particles, comprising:

a cartridge with a processing chamber in which the medium can be provided;

a multipole magnetic field generator with at least four subunits, each subunit having a core with a top surface and a coil surrounding said core, wherein the top surfaces of all subunits are arranged adjacent to the cartridge;

a control unit by which individual drive currents can be supplied to the subunits of the multipole magnetic field generator, comprising drive currents by which

a) magnetic particles are manipulated to mix a medium in the processing chamber;

b) magnetic particles are attracted to a surface of the processing chamber.

3. The processing device according to claim 2,

characterized in that the at least four subunits of the multipole magnetic field generator are arranged below the processing chamber and/or surround the processing chamber.

4. The processing device according to claim 2,

characterized in that an additional magnetic field generator is arranged opposite to the multipole magnetic field generator with respect to the processing chamber.

6. The processing device according to claim 2,

characterized in that the magnetic field comprises at least one oscillating component and/or that it is at least partially rotating.

7. The processing device according to claim 2,

characterized in that at least some of the magnetic particles comprise binding sites that specifically bind to target components of the medium.

8. The processing device according to claim 2,

characterized in that that at least some of the magnetic particles are capable of electrostatically binding particles, particularly fragments of nucleic acids.

9. The processing device according to claim 2,

characterized in that that at least some of the magnetic particles comprise silica.

17. Use of the processing device according to claim 2 for mixing a medium by actuating magnetic particles, for the separation of target components from a medium, and/or for molecular diagnostics, biological sample analysis, nucleic acid processing, chemical sample analysis, food analysis, and/or forensic analysis.

5. (canceled)

14. (canceled)

16. A processing device for processing a medium comprising target particles, comprising:

a cartridge with a processing chamber in which the medium can be provided;

a multipole magnetic field generator with at least four subunits, each subunit having a core with a top surface and a coil surrounding said core, wherein the top surfaces of all subunits are arranged adjacent to the cartridge;

an additional magnetic field generator that is arranged opposite to the multipole magnetic field generator with respect to the processing chamber;

a control unit by which individual drive currents can be supplied to the subunits of the multipole magnetic field generator and/or to the additional magnetic field generator.