METHODS AND SYSTEMS FOR DETECTION OF TARGET AND APPLICATIONS THEREOF
First Claim
Patent Images
1. A method of recovering a target from a sample, comprising:
- providing a substrate coupled binding element, wherein the binding element is immobilized on a solid support;
adding the sample comprising the target to the substrate coupled binding element to form a substrate coupled binding element-target complex;
washing the substrate coupled binding element-target complex; and
eluting the target from the substrate coupled binding element-target complex.
1 Assignment
0 Petitions
Accused Products
Abstract
A method of recovering a target from a sample is provided. The method of recovering the target follows different steps. The steps include providing a binding element, wherein the binding elements are immobilized on a solid support, adding the sample comprising the target to the binding element to form a binding element-target complex; washing the binding element-target complex; and eluting the target from the binding element-target complex. The system for reversible detection of target in a range from 2 to 1,000,000 bind/release cycles is also provided.
-
Citations
25 Claims
-
1. A method of recovering a target from a sample, comprising:
-
providing a substrate coupled binding element, wherein the binding element is immobilized on a solid support; adding the sample comprising the target to the substrate coupled binding element to form a substrate coupled binding element-target complex; washing the substrate coupled binding element-target complex; and eluting the target from the substrate coupled binding element-target complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
-
13. A method for reversible detection of a target, comprising:
-
immobilizing one or more binding elements on a sensor surface, contacting a sample comprising an unknown concentration of the target to the binding elements on the sensor surface to form a binding element-target complex in a target binding event; detecting the target-binding event with a detector to generate a detection signal; eluting the target from the binding element-target complex in an elution event; applying a regeneration solution to complete the removal of the target bound to the binding element in a regeneration event; determining a number of the target-binding event, the elution event, regeneration event or combinations thereof; and applying a correction to the detection signal based on the number of the binding event, the elution event, the regeneration event or combinations thereof during the reversible detection of the target over at least 100 bind/release cycles.
-
-
14. A method for reversible detection of a target and quantitation of a target concentration in a sample, comprising:
-
immobilizing one or more binding elements on a sensor surface, contacting a sample comprising the target to the binding elements to form a binding element-target complex in a target binding event; detecting the target-binding event with a detector to generate a detection signal; eluting the target from the binding element-target complex in an elution event; applying a regeneration solution to regenerate the binding element in a regeneration event; determining a number of the target binding event, the elution event, the regeneration event or combinations thereof; and applying a correction to the detection signal based on the number of the target binding event, the elution event, the regeneration event or combinations thereof during the reversible detection of the target and quantitation of the target concentration over at least 100 bind/release cycles. - View Dependent Claims (15, 16, 17, 18)
-
-
19. A system for reversible detection of a target, comprising:
-
a sample holder comprising one or more binding elements immobilized on a surface, wherein the surface is configured to load a sample comprising the target to complete a binding event forming a binding element-target complex; an inlet to supply at least a first solution to wash the binding element-target complex; an inlet to supply at least a second solution to elute the target from the binding element-target complex to complete an elution event; a detector to generate a detection signal in proportion to the concentration of the target in the sample; and a processor for calibrating performance of the system for reversible operation of the immobilized binding elements. - View Dependent Claims (20, 21, 22, 23, 24)
-
-
25. A system for reversible detection of a target, comprising:
-
a sample holder comprising one or more binding elements immobilized on a surface of the sample holder, wherein the surface is configured to load a sample comprising the target to form a binding element-target complex to complete a binding event; an inlet to supply at least a first solution to wash the binding element-target complex to complete a washing event; an inlet to supply at least a second solution to elute the target from the binding element-target complex to complete an elution event; a target-concentration detector to generate a detection signal in proportion to a concentration of the target in the sample; a counter for counting the binding event, washing event, elution event or combinations thereof; and a processor for calibrating a performance of the system based on a number of prior performed bind-release cycles for accurate reversible operation of the immobilized binding elements.
-
Specification