RNA-INTERFERENCE BY SINGLE-STRANDED RNA MOLECULES
First Claim
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16-1. The RISC according to claim 16, wherein said sulfur replaced monophosphate, diphosphate or triphosphate is 5′
- -alpha-thiotriphosphate or 5′
-gamma-thiotriphosphate, said 5′
-alkylphosphonate is RP(OH)(O)-0-5′
- or (OH)2(O)P-5′
-CH2—
), and said 5′
-alkyletherphosphonate is RP(OH)(O)—
O-5′
-.
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Abstract
The present invention relates to sequence and structural features of single-stranded (ss)RNA molecules required to mediate target-specific nucleic acid modifications by RNA-interference (RNAi), such as target mRNA degradation and/or DNA methylation.
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Citations
17 Claims
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16-1. The RISC according to claim 16, wherein said sulfur replaced monophosphate, diphosphate or triphosphate is 5′
- -alpha-thiotriphosphate or 5′
-gamma-thiotriphosphate, said 5′
-alkylphosphonate is RP(OH)(O)-0-5′
- or (OH)2(O)P-5′
-CH2—
), and said 5′
-alkyletherphosphonate is RP(OH)(O)—
O-5′
-.
- -alpha-thiotriphosphate or 5′
-
17-2. The RISC according to claim 1, wherein said RISC has a molecular weight of 120-160 kDa.
Specification