Feature Intensity Reconstruction of Biological Probe Array
First Claim
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1. A method of modifying image data comprising:
- theoretical pixel intensity with a transfer function representing the proportion of the intensity in a set of pixels due to a feature, a feature intensity of said feature, and a background value of said feature;
c. determining an optimized multiplicative error function using a multiplicative error function and a standard deviation function, wherein said multiplicative error function comprises said theoretical pixel intensity and said set of observed pixel values; and
d. determining said feature intensity with an update rule for said feature intensity using a weight function representing the weight of said pixel and the optimized multiplicative error function where said update rule for said feature intensity iteratively generates a unique value to which said feature intensity converges.obtaining an observed pixel intensity chart comprised of cells including a plurality a pixels for each cell wherein each pixel has an observed pixel intensity and wherein each cell has a center pixel,producing a feature intensity chart by averaging the pixel intensities of the pixels within each cell,producing a transfer function intensity chart having pixel intensities calculated using a transfer function that relates the distance of each pixel from the center pixel with an intensity value that is proportional to the observed feature intensity,multiplying the transfer function intensity chart by the feature intensity chart to form product image files, andsumming the product image files together to produce a summed image file.
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Abstract
The invention provides methods and systems for reconstructing feature intensities from pixel level data. In certain embodiments, the invention uses an empirically determined transfer function to construct a theoretical estimate of pixel level data and then iteratively updates feature intensities based on a minimum multiplicative error between the pixel level data and the theoretical estimate of the pixel level data.
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Citations
20 Claims
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1. A method of modifying image data comprising:
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theoretical pixel intensity with a transfer function representing the proportion of the intensity in a set of pixels due to a feature, a feature intensity of said feature, and a background value of said feature; c. determining an optimized multiplicative error function using a multiplicative error function and a standard deviation function, wherein said multiplicative error function comprises said theoretical pixel intensity and said set of observed pixel values; and d. determining said feature intensity with an update rule for said feature intensity using a weight function representing the weight of said pixel and the optimized multiplicative error function where said update rule for said feature intensity iteratively generates a unique value to which said feature intensity converges. obtaining an observed pixel intensity chart comprised of cells including a plurality a pixels for each cell wherein each pixel has an observed pixel intensity and wherein each cell has a center pixel, producing a feature intensity chart by averaging the pixel intensities of the pixels within each cell, producing a transfer function intensity chart having pixel intensities calculated using a transfer function that relates the distance of each pixel from the center pixel with an intensity value that is proportional to the observed feature intensity, multiplying the transfer function intensity chart by the feature intensity chart to form product image files, and summing the product image files together to produce a summed image file. - View Dependent Claims (2, 3, 4, 5)
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6. A computer program product for modifying image data comprising a processor to perform
obtaining an observed pixel intensity chart comprised of cells including a plurality a pixels for each cell wherein each pixel has an observed pixel intensity and wherein each cell has a center pixel, producing a feature intensity chart by averaging the pixel intensities of the pixels within each cell, producing a transfer function intensity chart having pixel intensities calculated using a transfer function that relates the distance of each pixel from the center pixel with an intensity value that is proportional to the observed feature intensity, multiplying the transfer function intensity chart by the feature intensity chart to form product image files, and summing the product image files together to produce a summed image file.
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11. A system for modifying image data comprising a processor and memory coupled to the processor with the memory storing a plurality of machine instructions that cause the processor to perform logical steps, wherein the logical steps comprise
obtaining an observed pixel intensity chart comprised of cells including a plurality a pixels for each cell wherein each pixel has an observed pixel intensity and wherein each cell has a center pixel, producing a feature intensity chart by averaging the pixel intensities of the pixels within each cell, producing a transfer function intensity chart having pixel intensities calculated using a transfer function that relates the distance of each pixel from the center pixel with an intensity value that is proportional to the observed feature intensity, multiplying the transfer function intensity chart by the feature intensity chart to form product image files, and summing the product image files together to produce a summed image file.
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16. A computer readable medium comprising computer-executable instructions for performing a method of modifying image data comprising
obtaining an observed pixel intensity chart comprised of cells including a plurality a pixels for each cell wherein each pixel has an observed pixel intensity and wherein each cell has a center pixel, producing a feature intensity chart by averaging the pixel intensities of the pixels within each cell, producing a transfer function intensity chart having pixel intensities calculated using a transfer function that relates the distance of each pixel from the center pixel with an intensity value that is proportional to the observed feature intensity, multiplying the transfer function intensity chart by the feature intensity chart to form product image files, and summing the product image files together to produce a summed image file, wherein the medium is selected from the group consisting of CD-ROM, floppy disk, tape, flash memory, system memory and hard drive.
Specification