Compositions and Methods Related to CRISPR Targeting
First Claim
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1. A composition comprising:
- an non-integrating epichromosomal vector encoding at least one of a cas gene, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), or CRISPR guide RNA;
one or more target sequences, andone or more condition-inducible promoters.
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Abstract
Disclosed herein include embodiments related to addition, deletion, or modification of DNA, RNA, or protein in a subject. In an embodiment, the DNA, RNA, or protein is endogenous. In an embodiment, the DNA, RNA, or protein is exogenous. Further embodiments relate to computerized systems for assisting in the disclosed methods.
235 Citations
45 Claims
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1. A composition comprising:
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an non-integrating epichromosomal vector encoding at least one of a cas gene, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), or CRISPR guide RNA; one or more target sequences, and one or more condition-inducible promoters. - View Dependent Claims (2, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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3-13. -13. (canceled)
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24. (canceled)
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25. A method, comprising:
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administering to a host cell, a non-integrating epichromosome vector encoding at least one of a cas gene, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), or CRISPR guide RNA; one or more target sequences, and a condition-inducible promoter. - View Dependent Claims (32, 33, 34)
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26-31. -31. (canceled)
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35. A method, comprising:
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inhibiting a pathogen by administering to a host cell, a non-integrating epichromosome vector encoding at least one of a cas gene, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), or CRISPR guide RNA; one or more target sequences of a pathogenic antigen against which an immune response is desired, and a condition-inducible promoter.
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36-37. -37. (canceled)
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38. A method, comprising:
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reducing or eliminating an immune response to an antigen against which an immune response is not desired by administering to a host cell, a non-integrating epichromosome vector encoding at least one of a cas gene, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), or CRISPR guide RNA; one or more target sequences of the antigen against which an immune response is not desired, and a condition-inducible promoter. - View Dependent Claims (39)
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40. A method, comprising:
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in vivo genetic editing by administering to a host cell, a non-integrating epichromosome vector encoding at least one of a cas gene, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), or CRISPR guide RNA; one or more target sequences for desired editing, and a condition-inducible promoter.
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41-42. -42. (canceled)
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43. A method, comprising:
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reducing or eliminating an immune response to an antigen against which an immune response is not desired by administering to a host lymphocyte cell, a non-integrating epichromosome vector encoding at least one of a cas gene, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), or CRISPR guide RNA; and one or more target sequences of at least one lymphocyte receptor variable chain sequence against which an immune response is not desired. - View Dependent Claims (44, 45)
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Specification