BACTERIAL COMPOSITION

• US 20150104418A1
• Filed: 12/18/2014
• Published: 04/16/2015
• Est. Priority Date: 12/18/2014
• Status: Abandoned Application

• Current Assignee: MicroBios Inc.
• Original Assignee: MicroBios Inc.
• Inventors: Garner, Matthew Ryan, Flint, Joseph F.
• Application Number:
  US14/575,916
• Publication Number:
  US 20150104418A1
• Time in Patent Office: Days
• US Class Current: 424/93.3
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23K 50/20 for horses
• CPC Class Codes: A23K 50/30 for swines
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23V 2400/117 Animalis
• CPC Class Codes: A23V 2400/413 Acidilactici
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 35/744 Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis

Abstract

A bacterial composition that inhibits E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains referred to as the non-O157 STECs that include E. coli O121:H19; E. coli O45:H2; E. coli O103:H11; E. coli O145, E. coli O26:H11; and E. coli O111. The composition is constituted of various combinations of the following four unique pathogen-inhibiting bacteria: (1) Lactobacillus animalis strain MB101; (2) Lactobacillus animalis strain MB102; (3) Enterococcus faecium strain MB505; and (4) Pediococcus acidilactici strain MB902. Each of the discovered bacteria is deposited with the American Type Culture Collection (ATCC) and respectively has an individual ATCC Accession Number.

Claims

1-26. -26. (canceled)

27. A method for preparing a probiotic composition comprising:

mixing into a combination at least two bacteria selected from the group consisting of;

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710;

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711;

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and

packaging the combination.

28. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; and

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711.

29. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710;

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; and

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709.

30. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710;

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711;

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712.

31. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; and

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709.

32. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710;

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712.

33. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712.

34. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB101 having ATCC Accession Number PTA-121710;

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712.

35. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; and

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709.

36. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711;

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712.

37. The method recited in claim 27 comprising mixing:

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712.

38. The method recited in claim 27 comprising mixing:

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712.

39. The method recited in claim 27 wherein at least one strain is a pathogen growth inhibitor.

40. The method recited in claim 27 wherein at least one strain is an E. coli O157:

H7 growth inhibitor.

41. The method recited in claim 27 wherein at least one strain is a growth inhibitor to the non-O157 STEC, Big-Six Escherichia coli strains including E. coli O121:

H19;

E. coli O45;

H2;

E. coli O103;

H11;

E. coli O145, E. coli O26;

H11; and

E. coli O111.

42. The method recited in claim 27 wherein at least one strain is a Salmonella growth inhibitor.

43. The method recited in claim 27 wherein at least one strain is a Salmonella typhimurium growth inhibitor.

44. The method recited in claim 27 wherein at least one strain is a Salmonella enteriditis growth inhibitor.

45. The method recited in claim 27 wherein each strain is a pathogen growth inhibitor.

46. The method recited in claim 27 wherein each strain is an E. coli O157:

H7 growth inhibitor.

47. The method recited in claim 27 wherein each strain is a growth inhibitor to the non-O157 STEC, Big-Six Escherichia coli strains including E. coli O121:

H19;

E. coli O45;

H2;

E. coli O103;

H11;

E. coli O145, E. coli O26;

H11; and

E. coli O111.

48. The method recited in claim 27 wherein each strain is a Salmonella growth inhibitor.

49. The method recited in claim 27 wherein each strain is a Salmonella typhimurium growth inhibitor.

50. The method recited in claim 27 wherein at least one strain is a Salmonella enteriditis growth inhibitor.

51. The method recited in claim 27 further comprises mixing in a carrier and thereby establishing a direct fed bacterial animal feed additive.

52. A method for preparing a probiotic composition comprising:

obtaining at least one bacteria selected from the group consisting of;

Lactobacillus animalis strain MB102 having ATCC Accession Number PTA-121711;

Enterococcus faecium strain MB505 having ATCC Accession Number PTA-121709; and

Pediococcus acidilactici strain MB902 having ATCC Accession Number PTA-121712; and

packaging the composition.