Methods and Compositions for Generating Pancreatic Progenitors and Functional Beta Cells from hPSCs
First Claim
1. A method of producing NKX6-1+ pancreatic progenitor cells from an endodermal cell population, the method comprising contacting the endodermal cell population with an EGF component, a Nicotinamide component and/or a Noggin component, optionally a combination of at least one EGF component and at least one nicotinamide component to induce the differentiation of at least a portion of the endodermal cell population into NKX6-1+ pancreatic progenitor cells, the combination optionally further comprising at least one Noggin component.
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Accused Products
Abstract
Methods and compositions for producing NKX6-1+ pancreatic progenitor cells and/or insulin producing cells from an endodermal cell population, the method comprising contacting the endodermal cell population with an EGF component, a Nicotinamide component and/or a Noggin component, optionally a combination of at least one EGF component and at least one nicotinamide component to induce the differentiation of at least one endodermal cell into a NKX6-1+ pancreatic progenitor cell, the combination optionally further comprising at least one Noggin component.
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Citations
29 Claims
- 1. A method of producing NKX6-1+ pancreatic progenitor cells from an endodermal cell population, the method comprising contacting the endodermal cell population with an EGF component, a Nicotinamide component and/or a Noggin component, optionally a combination of at least one EGF component and at least one nicotinamide component to induce the differentiation of at least a portion of the endodermal cell population into NKX6-1+ pancreatic progenitor cells, the combination optionally further comprising at least one Noggin component.
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3. (canceled)
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6. (canceled)
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8. (canceled)
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11. (canceled)
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13. (canceled)
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18. (canceled)
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21. (canceled)
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25. (canceled)
- 26. A culture medium supplement composition comprising an EGF component, a Nicotinamide component and optionally a Noggin component and/or an Exendin-4 component.
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28. A kit comprising:
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an EGF component, a Nicotinamide component; and
/ora Noggin component and/or optionally an Exendin-4 component.
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29. (canceled)
Specification