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Assembly of Nucleic Acid Sequences in Emulsions

  • US 20150111256A1
  • Filed: 02/14/2013
  • Published: 04/23/2015
  • Est. Priority Date: 02/17/2012
  • Status: Active Grant
First Claim
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1. A method of synthesizing a target nucleic acid sequence comprising the steps of:

  • localizing a plurality of oligonucleotide subsequences defining an oligonucleotide set corresponding to a particular target nucleic acid sequence by hybridization to a predesigned sequence that is unique to each oligonucleotide set;

    attaching the plurality of oligonucleotide subsequences to a bead;

    placing the bead within an emulsion droplet;

    separating the plurality of oligonucleotide subsequences from the bead such that the plurality of oligonucleotide subsequences remain within the emulsion droplet; and

    assembling within the emulsion droplet the plurality of oligonucleotide subsequences to form the target nucleic acid sequence.

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