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Digital Counting of Individual Molecules by Stochastic Attachment of Diverse Labels

  • US 20150118680A1
  • Filed: 07/08/2014
  • Published: 04/30/2015
  • Est. Priority Date: 12/15/2009
  • Status: Abandoned Application
First Claim
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1. A method comprising:

  • a) amplifying a population of different target DNA molecules from a tagged genomic sample thereby producing a population of amplified target DNA molecules,wherein the different target DNA molecules that comprise a polymorphic site are tagged with different label-tags, wherein the label-tags comprise nucleotides selected from purine bases, pyrimidine bases, natural nucleotide bases, chemically modified nucleotide bases, biochemically modified nucleotide bases, non-natural nucleotide bases and derivatized nucleotide bases; and

    wherein each of the population of amplified target DNA molecules comprises the polymorphic site and an associated label-tag of the different label-tags; and

    b) detecting the plurality of amplified target DNA molecules by sequencing, thereby producing a plurality of readouts, wherein the plurality of readouts comprises;

    (i) at least a portion of the target region; and

    (ii) an associated label-tag of the label-tags.

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