APPARATUS AND METHOD FOR SEPARATING A BIOLOGICAL ENTITY FROM A SAMPLE VOLUME
First Claim
1. An apparatus for separating a biological entity from a sample volume, the apparatus comprising:
- an input chamber comprising;
an inlet configured to receive the sample volume; and
an outlet;
at least one magnetic element adjacent a portion of the input chamber, the magnetic element configured to provide a magnetic field in a vicinity of the portion of the input chamber to trap at least some leukocytes from the sample volume; and
a filter in fluid communication with the outlet, the filter configured to separate the biological entity.
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Accused Products
Abstract
According to embodiments of the present invention, an apparatus for separating a biological entity from a sample volume is provided. The apparatus includes an input chamber including an inlet configured to receive the volume sample, and an outlet, at least one magnetic element adjacent a portion of the input chamber, the magnetic element configured to provide a magnetic field in a vicinity of the portion of the input chamber to trap at least some leukocytes from the sample volume, and a filter in fluid communication with the outlet, the filter configured to separate the biological entity. According to further embodiments of the present invention, a method for separating a biological entity from a sample volume is also provided.
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Citations
34 Claims
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1. An apparatus for separating a biological entity from a sample volume, the apparatus comprising:
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an input chamber comprising; an inlet configured to receive the sample volume; and an outlet; at least one magnetic element adjacent a portion of the input chamber, the magnetic element configured to provide a magnetic field in a vicinity of the portion of the input chamber to trap at least some leukocytes from the sample volume; and a filter in fluid communication with the outlet, the filter configured to separate the biological entity. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 24)
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4. (canceled)
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11. (canceled)
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14. (canceled)
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15. (canceled)
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22. (canceled)
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23. (canceled)
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25-28. -28. (canceled)
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29. A method for separating a biological entity from a sample volume, the method comprising:
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supplying the sample volume to an input chamber; supplying a plurality of magnetic beads to the input chamber, the plurality of magnetic beads couplable to leukocyte specific biomarkers; trapping leukocytes from the sample volume that are coupled to the plurality of magnetic beads at a portion of the input chamber via at least one magnetic element; and filtering the sample volume by means of a filter for separating the biological entity.
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30-32. -32. (canceled)
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33. A method for separating a biological entity from a sample volume, the method comprising:
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supplying the sample volume to an input chamber; filtering the sample volume by means of a filter for trapping the biological entity and at least some of leukocytes, fetal cells or stem cells on the filter; supplying a plurality of magnetic beads couplable to leukocyte specific biomarkers to the filter; flowing the trapped contents of the filter into the input chamber; trapping the leukocytes from the sample volume that are coupled to the plurality of magnetic beads at a portion of the input chamber via at least one magnetic element; and filtering the sample volume by means of the filter for separating the biological entity.
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34. (canceled)
Specification