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NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES

  • US 20150119259A1
  • Filed: 04/08/2013
  • Published: 04/30/2015
  • Est. Priority Date: 06/20/2012
  • Status: Abandoned Application
First Claim
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1. A method for sequencing a nucleic acid molecule, the method comprising:

  • (a) providing a chip comprising a plurality of individually addressable nanopores, wherein an individually addressable nanopore of said plurality of individually addressable nanopores comprises a nanopore in a membrane that is disposed adjacent to an electrode, wherein said nanopore is linked to a nucleic acid polymerase, and wherein each individually addressable nanopore is adapted to detect a tag that is released from a tagged nucleotide upon the polymerization of said tagged nucleotide;

    (b) directing said nucleic acid molecule adjacent to or in proximity to said nanopore;

    (c) with the aid of said polymerase, polymerizing nucleotides along said nucleic acid molecule to generate a strand that is complementary to at least a portion of said nucleic acid molecule, wherein during polymerization a tag is released from an individual nucleotide of said nucleotides, and wherein said released tag flows through or in proximity to said nanopore; and

    (d) detecting the tag with the aid of said electrode, wherein the tag is detected subsequent to being released from said individual nucleotide.

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