BISPECIFIC ANTIBODY MOLECULE
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Abstract
The present invention relates to a bispecific antibody molecule, as well as a method for producing the same, its use and a nucleic acid molecule encoding the bispecific antibody molecule. The invention in particular provides an antibody molecule that is capable of mediating target cell restricted activation of immune cells.
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Citations
80 Claims
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1-57. -57. (canceled)
- 58. A recombinant bispecific antibody molecule consisting of a Fab fragment comprising a first binding site for a first antigen, a single chain Fv fragment comprising a second binding site for a second antigen and an immunoglobulin CH2 domain, wherein the Fab fragment and the single chain Fv fragment are linked via the CH2 domain, wherein at least one amino acid residue of the CH2 domain that is able to mediate binding to Fc receptors is lacking or mutated, and wherein further one or more amino acid residues of sequence positions 226, 228 and 229, is lacking or mutated.
- 74. A recombinant bispecific antibody molecule consisting of a Fab fragment comprising a first binding site for a first antigen, a single chain Fv fragment comprising a second binding site for a second antigen, an immunoglobulin CH2 domain, and an immunoglobulin CH3 domain, wherein the Fab fragment and the single chain Fv fragment are linked to each other via the CH2 domain and CH3 domain, and wherein at least one amino acid residue of the CH2 domain that is able to mediate binding to an Fc-receptor is lacking or mutated, wherein the at least one amino acid residue of the CH2 domain that is able to mediate binding to an Fc-receptor is lacking or mutated is selected from the group consisting of sequence positions 228, 230, 231, 232, 233, 234, 235, 236, 237, 238, 265, 297, 327 and 330 (numbering of sequence positions according to the EU-index).
Specification